Dentin matrix stabilization following treatment with different crosslinking agents: Effect on dentin bonding.

• Grant number: 08/06170-7
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): October 01, 2008
• Effective date (End): March 31, 2011
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal researcher: Jose Carlos Pereira
• Grantee: Renato Cilli
• Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

In order to bond to dentin by formation of the hybrid-layer most of the current adhesive systems rely on Kanca III's wet bonding technique in which water plays an important role. Water stabilizes demineralized dentin matrix in an expanded state thus allowing hydrophilic monomers to occupy the porosities left by acid demineralization. As dentin wetness control during bonding is a critical issue, residual water may hump resin infiltration or chemically combine with adhesive monomers, therefore leading to structural problems such as bonding deficiency, nanoleakage and premature degradation. The aim of the present research project is to investigate the treatment of demineralized dentin matrix with different crosslinking agents and their role on resin-bonding to relatively dry dentinal surface. They comprise natural (proanthocyanidin and genipin) and synthetic (glutaraldehyde) crosslinkers, and dentin bonding primers will be formulated with these agents. Human third molars will be selected for bonding and demineralized dentin will be treated with the different crosslinking primers prior to bond with an experimental adhesive system. Specimens will be evaluated for bond strength by the microtensile method and data will be subjected to statistical analysis with 5% of significance level. In addition, specimens will be prepared to scanning electron microscopy for hybrid layer examination in respect to identify collagen fibrils not enveloped by adhesive resin. The one-year time-effect on bonding using the different collagen crosslinkers will be also evaluated.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CILLI, RENATO; PEREIRA, JOSE CARLOS; PRAKKI, ANURADHA. Properties of dental resins submitted to pH catalysed hydrolysis. Journal of Dentistry, v. 40, n. 12, p. 1144-1150, DEC 2012. (08/06170-7)

PALLAN, SMILEY; ARAUJO, MICHEL VICTOR FURTADO; CILLI, RENATO; PRAKKI, ANURADHA. Mechanical properties and characteristics of developmental copolymers incorporating catechin or chlorhexidine. Dental Materials, v. 28, n. 6, p. 687-694, JUN 2012. (08/06170-7)

CILLI, RENATO; RIBEIRO DE MATTOS, MARIA CECILIA; HONORIO, HEITOR MARQUES; RIOS, DANIELA; DE ARAUJO, PAULO AMARANTE; PRAKKI, ANURADHA. The role of surface sealants in the roughness of composites after a simulated toothbrushing test. Journal of Dentistry, v. 37, n. 12, p. 970-977, DEC 2009. (08/06170-7)