Increased osmolality enhances the tight junction-mediated barrier function in a cultured renal epithelial cell line

Autor(es)

Canuto, Leandro P. ^[1] ; Collares-Buzato, Carla B. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cell Biology International; v. 43, n. 1, p. 73-82, JAN 2019.
• Citações Web of Science: 1

Resumo

Osmotic alterations are associated with several human diseases, including diabetic nephropathy. We have previously shown that high glucose, which is a well-known osmotic agent, induces significant disruption of the tight junction (TJ)-mediated tubular barrier of the Madin-Darby canine kidney (MDCK) cell line. In this study, we investigated the effect of acute (24 h) and chronic (72 h) exposure to increased osmolality (with a 14.5 mM mannitol solution) on TJ-mediated barrier function in MDCK cells. The treatment with mannitol significantly increased the transepithelial electrical resistance (TEER) and accelerated the TEER recovery after Ca2+ switch assay in comparison with control monolayers. Immunofluorescence and Western blot analyses showed that mannitol treatment induced a significant increase in the tight junctional and cellular content of claudin-1 (a barrier-forming claudin) as well as a significant decrease in claudin-2 (a pore-forming claudin) junctional and cellular contents. These data suggest that an increased osmolality induces enhancement of the TJ-mediated barrier of MDCK cells, and that, therefore, the negative effect of high glucose on the epithelial paracellular barrier cannot be attributed to its osmotic actions. In addition, a subtle increase in osmolality may have an impact on kidney function and renal-related diseases. (AU)

• Processo FAPESP: 13/15676-0 - Modulação in vitro da barreira epitelial intestinal mediada pelas junções de oclusão (tight junctions): efeito da exposição a nutrientes (glicose e ácidos graxos), à insulina, e à microflora bacteriana de animais pré-diabéticos
• Beneficiário: Carla Beatriz Collares Buzato
• Modalidade de apoio: Auxílio à Pesquisa - Regular