Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Increased osmolality enhances the tight junction-mediated barrier function in a cultured renal epithelial cell line

Full text
Author(s):
Canuto, Leandro P. [1] ; Collares-Buzato, Carla B. [1]
Total Authors: 2
Affiliation:
[1] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Cell Biology International; v. 43, n. 1, p. 73-82, JAN 2019.
Web of Science Citations: 1
Abstract

Osmotic alterations are associated with several human diseases, including diabetic nephropathy. We have previously shown that high glucose, which is a well-known osmotic agent, induces significant disruption of the tight junction (TJ)-mediated tubular barrier of the Madin-Darby canine kidney (MDCK) cell line. In this study, we investigated the effect of acute (24 h) and chronic (72 h) exposure to increased osmolality (with a 14.5 mM mannitol solution) on TJ-mediated barrier function in MDCK cells. The treatment with mannitol significantly increased the transepithelial electrical resistance (TEER) and accelerated the TEER recovery after Ca2+ switch assay in comparison with control monolayers. Immunofluorescence and Western blot analyses showed that mannitol treatment induced a significant increase in the tight junctional and cellular content of claudin-1 (a barrier-forming claudin) as well as a significant decrease in claudin-2 (a pore-forming claudin) junctional and cellular contents. These data suggest that an increased osmolality induces enhancement of the TJ-mediated barrier of MDCK cells, and that, therefore, the negative effect of high glucose on the epithelial paracellular barrier cannot be attributed to its osmotic actions. In addition, a subtle increase in osmolality may have an impact on kidney function and renal-related diseases. (AU)

FAPESP's process: 13/15676-0 - In vitro modulation of intestinal epithelial barrier mediated by tight junctions: effect of exposure to nutrients (glucose and fatty acids), insulin and bacterial microbiota of prediabetic animals
Grantee:Carla Beatriz Collares Buzato
Support Opportunities: Regular Research Grants