Frequent detection of CXCR4-using viruses among Brazilian blood donors with HIV-1 long-standing infection and unknown clinical stage: Analysis of massive parallel sequencing data

Autor(es)

Pessoa, Rodrigo ^[1] ; Sanabani, Sabri S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Hosp Clin, Dept Pathol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: DATA IN BRIEF; v. 6, p. 267-274, MAR 2016.
• Citações Web of Science: 0

Resumo

The determination of viral tropism is critically important and highly recommended to guide therapy with the CCR5 antagonist, which does not inhibit the effect of X4-tropic viruses. Here, we report the prevalence of HIV-1 x 4 HIV strains in 84 proviral DNA massively parallel sequencing ``MPS{''} data from well-defined non-recently infected first-time Brazilian blood donors. The MPS data covering the entire V3 region of the env gene was extracted from our recently generated HIV-1 genomes sequenced by a paired-end protocol (Illumina). Of the 84 MPS data samples, 63 (75%) were derived from donors with long-standing infection and 21 (25%) were lacking stage information. HIV-1 tropism was inferred using Geno2pheno (g2p) ({[}454]) algorithm (FPR = 1%, 2.5%, and 3.75%). Among the 84 data samples for which tropism was defined by g2p(2.5%), 13 (15.5%) participants had detectable CXCR4-using viruses in their MPS reads. Mixed infections with R5 and X4 were observed in 11.9% of the study subjects and minority X4 viruses were detected in 7 (8.3%) of participants. Nine of the 63 (14.3%) subjects with LS infection were predicted by g2p (2.5%) to harbor proviral CXCR4-using viruses. Our findings of a high proportion of blood donors (15.5%) harboring CXCR4-using viruses in PBMCs may indicate that this phenomenon is common. These findings may have implications for clinical and therapeutic aspects and may benefit individuals who plan to receive CCR5 antagonists. (C) 2015 The Authors. Published by Elsevier Inc. (AU)

• Processo FAPESP: 14/24596-2 - Sequenciamento em larga escala dos genomas do HIV na forma de RNA livre e DNA integrado: comparação entre os subtipos gerados, mutações relacionadas a resistência de drogas e o uso de co-receptores
• Beneficiário: Rodrigo Pessoa de Farias
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/12297-2 - Análise dos transcritos de miRNAs, REX e tax em células T no curso da infecção pelo HTLV-1, utilizando sequenciamento de nova geração
• Beneficiário: Sabri Saeed Mohammed Ahmed Al-Sanabani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/11090-5 - Caracterização do vírus da imunodeficiência humana (HIV-1) pela análise do genoma completo viral em amostras de doadores de sangue nos estados de Pernambuco, Minas Gerais, Rio de Janeiro e São Paulo
• Beneficiário: Sabri Saeed Mohammed Ahmed Al-Sanabani
• Modalidade de apoio: Auxílio à Pesquisa - Regular