Analysis of the effects of mesoporous silica particles SBA-15 and SBA-16 in Streptococcus pneumoniae transformation process

Autor(es)

Amstalden, Maria-Cecilia Krahenbuehl ^{[1, 2]} ; Oliveira, Jose Diogo ^[1] ; Strauss, Mathias ^{[3, 4]} ; Mazali, Italo Odone ; Machado, Daisy ^[1] ; Theisen, Thais Holtz ^[1] ; Lancellotti, Marcelo ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Fac Pharmaceut Sci, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Inst Chem, Funct Mat Lab, Campinas, SP - Brazil

^[4] Natl Ctr Res Energy & Mat, Brazilian Nanotechnol Natl Lab, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Folia Microbiologica; v. 64, n. 1, p. 127-132, JAN 2019.
• Citações Web of Science: 0

Resumo

Streptococcus pneumoniae are natural competent bacteria which requires the presence of a pheromone-like molecule to do the transformation process. This study verified the influence of mesoporous silica (SBA-15 and SBA-16) on the transformation process in S. pneumoniae using a donor DNA obtained from a mutant strain of this microorganism (Sp360luxS). The results showed that mesoporous silica SBA-15 and SBA-16 particles doubled the transformation ratio frequency compared with negative control (without nanoparticles) in using SBA-15 (ratio 1.81 +/- 0.04) and SBA-16 (ratio 2.18 +/- 0.22). We demonstrated the how mesoporous silica nanoparticles were able to increase the pneumococcus transformations, which could possibly lead to the acquisition of virulence factor genes and resistance of antibiotics. (AU)

• Processo FAPESP: 14/50906-9 - INCT 2014: em Materiais Complexos Funcionais (INOMAT)
• Beneficiário: Fernando Galembeck
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/14079-2 - Uso de nanopartículas em vacinas contra meningites bacterianas
• Beneficiário: Marcelo Lancellotti
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/22127-2 - Desenvolvimento de novos materiais estratégicos para dispositivos analíticos integrados
• Beneficiário: Lauro Tatsuo Kubota
• Modalidade de apoio: Auxílio à Pesquisa - Temático