Analysis of the effects of mesoporous silica particles SBA-15 and SBA-16 in Streptococcus pneumoniae transformation process

Author(s)

Amstalden, Maria-Cecilia Krahenbuehl ^{[1, 2]} ; Oliveira, Jose Diogo ^[1] ; Strauss, Mathias ^{[3, 4]} ; Mazali, Italo Odone ; Machado, Daisy ^[1] ; Theisen, Thais Holtz ^[1] ; Lancellotti, Marcelo ^{[1, 2]}

Affiliation

^[1] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Fac Pharmaceut Sci, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Inst Chem, Funct Mat Lab, Campinas, SP - Brazil

^[4] Natl Ctr Res Energy & Mat, Brazilian Nanotechnol Natl Lab, Campinas, SP - Brazil

• Document type: Journal article
• Source: Folia Microbiologica; v. 64, n. 1, p. 127-132, JAN 2019.
• Web of Science Citations: 0

Abstract

Streptococcus pneumoniae are natural competent bacteria which requires the presence of a pheromone-like molecule to do the transformation process. This study verified the influence of mesoporous silica (SBA-15 and SBA-16) on the transformation process in S. pneumoniae using a donor DNA obtained from a mutant strain of this microorganism (Sp360luxS). The results showed that mesoporous silica SBA-15 and SBA-16 particles doubled the transformation ratio frequency compared with negative control (without nanoparticles) in using SBA-15 (ratio 1.81 +/- 0.04) and SBA-16 (ratio 2.18 +/- 0.22). We demonstrated the how mesoporous silica nanoparticles were able to increase the pneumococcus transformations, which could possibly lead to the acquisition of virulence factor genes and resistance of antibiotics. (AU)

• FAPESP's process: 14/50906-9 - INCT 2014: in Functional Complex Materials
• Grantee: Fernando Galembeck
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 11/14079-2 - Use of nanoparticles in vaccines against bacterial meningitis
• Grantee: Marcelo Lancellotti
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/22127-2 - Development of novel materials strategic for integrated analytical devices
• Grantee: Lauro Tatsuo Kubota
• Support Opportunities: Research Projects - Thematic Grants