| Texto completo | |
| Autor(es): |
Frediani, Mayra H.
[1]
;
Guida, Fernanda J. V.
[2]
;
Salgado, Paula A. B.
[2]
;
Goncalves, Debora R.
[3]
;
Blank, Marcel H.
[1]
;
Novaes, Gabriel A.
[1]
;
Pereira, Ricardo J. G.
[1]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Reprod Anim, GEMA, BR-05508900 Sao Paulo, SP - Brazil
[2] Fundacao Parque Zool Sao Paulo, BR-04301012 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Reprod Anim, Reprod Aves, Sao Paulo - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Theriogenology; v. 125, p. 140-151, FEB 2019. |
| Citações Web of Science: | 0 |
| Resumo | |
It is unquestinable that artificial insemination (Al) offers many benefits to avian conservation programs, but a serious impediment towards implementing AI for wild species is the development of effective techniques to consistently collect good quality ejaculates. Thus, we aimed to examine the success rate of electro-stimulation (ES) in collecting semen from 49 unconditioned males from orders Piciformes, Strigiformes, Accipitriformes, Cathartiformes, Galiformes, Anseriformes and Psittaciformes at different times of the year. Sixty out of 299 ES attempts provided ejaculates with sperm, but collection success rates varied widely (0-50%) depending on the species. Except for swans whose greater results were registered during spring-summer, males from most orders responded better to ES during winter-spring, suggesting seasonal variations on semen collection success rates. Overall, ES enabled successful semen collection from males of unproven and proven fertility under mixed pairing conditions. However, the highest success rate occurred in paired males with fertile clutches (40.6%) followed by unpaired males (22.1%), paired males without clutches (13.9%), and paired males with infertile clutches (6.8%). Behavioral responses of male birds to electrical impulses were also recorded to assess any discomfort during semen collection. Furthermore, macroscopic and microscopic analysis provided ejaculate parameters from several species, even from orders that hitherto have never been assessed for semen collection, which may serve as a starting point in the future. Altogether, these findings demonstrate the feasibility of ES in collecting semen from unpaired, unconditioned and non-imprinted males from a variety of bird orders. In the medium to long term, the use of this technique in both captive and free-ranging populations offers new perspectives to ensure genetic diversity in avian conservation programs. (C) 2018 Elsevier Inc. All rights reserved. (AU) | |
| Processo FAPESP: | 16/17158-4 - Conservação de germoplasma de cracídeos ameaçados por meio de transplantes interespecíficos de células germinativas |
| Beneficiário: | Ricardo Jose Garcia Pereira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 12/08621-1 - Conservação de germoplasma aviário via transplantes interespecíficos de espermatogônias: influência da idade dos receptores na resturação da espermatogênese pelas células doadoras |
| Beneficiário: | Ricardo Jose Garcia Pereira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |