Protein refolding based on high hydrostatic pressure and alkaline pH: Application on a recombinant dengue virus NS1 protein

Autor(es)

Chura-Chambi, Rosa Maria ^[1] ; Rosa da Silva, Cleide Mara ^[1] ; Pereira, Lennon Ramos ^[2] ; Bartolini, Paolo ^[1] ; de Souza Ferreira, Luis Carlos ^[2] ; Morganti, Ligia ^[1]

Afiliação do(s) autor(es)

^[1] IPEN CNEN SP, Inst Pesquisas Energet & Nucl, Ctr Biotecnol, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 14, n. 1 JAN 25 2019.
• Citações Web of Science: 3

Resumo

In this study we evaluated the association of high hydrostatic pressure (HHP) and alkaline pH as a minimally denaturing condition for the solubilization of inclusion bodies (IBs) generated by recombinant proteins expressed by Escherichia coli strains. The method was successfully applied to a recombinant form of the dengue virus (DENV) non-structural protein 1 (NS1). The minimal pH for IBs solubilization at 1 bar was 12 while a pH of 10 was sufficient for solubilization at HHP: 2.4 kbar for 90 min and 0.4 kbar for 14 h 30 min. An optimal refolding condition was achieved by compression of IBs at HHP and pH 10.5 in the presence of arginine, oxidized and reduced glutathiones, providing much higher yields (up to 8-fold) than association of HHP and GdnHCl via an established protocol. The refolded NS1, 109 +/- 9.5 mg/L bacterial culture was recovered mainly as monomer and dimer, corresponding up to 90% of the total protein and remaining immunologically active. The proposed conditions represent an alternative for the refolding of immunologically active recombinant proteins expressed as IBs. (AU)

• Processo FAPESP: 15/02574-0 - Renaturação de antígenos sintetizados como corpos de inclusão em Escherichia coli para preparação de vacinas
• Beneficiário: Ligia Ely Morganti Ferreira Dias
• Modalidade de apoio: Auxílio à Pesquisa - Regular