| Texto completo | |
| Autor(es): |
Juliana Soares Roter Marins
[1]
;
Luciana Moura Sassone
[2]
;
Sandra Rivera Fidel
[3]
;
Daniel Araki Ribeiro
[4]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] State University of Rio de Janeiro. Dental School. Proclin Department - Brasil
[2] State University of Rio de Janeiro. Dental School. Proclin Department - Brasil
[3] State University of Rio de Janeiro. Dental School. Proclin Department - Brasil
[4] Federal University of São Paulo. Dental School. Department of Bioscience - Brasil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Brazilian Dental Journal; v. 23, n. 5, p. 527-533, 2012-10-00. |
| Resumo | |
The aim of the present study was to evaluate the capacity of some root canal irrigants to induce genetic damage and/or cellular death in vitro. Murine fibroblast cells were exposed to ethylenediaminetetraacetic acid (EDTA), sodium hypochlorite (NaOCl), MTAD™ and citric acid in increasing concentrations for 3 h at 37ºC. The negative control group was treated with vehicle control (phosphate buffer solution - PBS) for 3 h at 37°C, and the positive control group was treated with methylmetanesulfonate, 1 μM. for 3 h at 37°C. Cytotoxicity was assessed by the trypan blue test and genotoxicity was evaluated by the single cell gel (comet) assay. The results showed that exposure to 2.5% and 5% NaOCl and 8.5% citric acid resulted in a significant cytotoxic effect. NaOCl, EDTA and citric acid did not produce genotoxic effects with respect to the comet assay data for all evaluated concentrations. Although MTAD was not a cytotoxic agent, it showed significant genotoxic effects at all tested concentrations (ANOVA and Tukey's test; p<0.05). NaOCl, EDTA and citric acid were found to be cytotoxic in a dose-dependent manner, but they were not genotoxic. MTAD did not cause cell death, but presented genotoxic effects. (AU) | |
| Processo FAPESP: | 07/01228-4 - Carcinogênese bucal quimicamente induzida pela 4-nitroquinolina 1-óxido em ratos: possíveis biomarcadores envolvidos em sua patogênese |
| Beneficiário: | Daniel Araki Ribeiro |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |