In vitro genotoxicity and cytotoxicity in murine fibroblasts exposed to EDTA, NaOCl, MTAD and citric acid

Autor(es)

Juliana Soares Roter Marins ^[1] ; Luciana Moura Sassone ^[2] ; Sandra Rivera Fidel ^[3] ; Daniel Araki Ribeiro ^[4]

Afiliação do(s) autor(es)

^[1] State University of Rio de Janeiro. Dental School. Proclin Department - Brasil

^[2] State University of Rio de Janeiro. Dental School. Proclin Department - Brasil

^[3] State University of Rio de Janeiro. Dental School. Proclin Department - Brasil

^[4] Federal University of São Paulo. Dental School. Department of Bioscience - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 23, n. 5, p. 527-533, 2012-10-00.

Resumo

The aim of the present study was to evaluate the capacity of some root canal irrigants to induce genetic damage and/or cellular death in vitro. Murine fibroblast cells were exposed to ethylenediaminetetraacetic acid (EDTA), sodium hypochlorite (NaOCl), MTAD™ and citric acid in increasing concentrations for 3 h at 37ºC. The negative control group was treated with vehicle control (phosphate buffer solution - PBS) for 3 h at 37°C, and the positive control group was treated with methylmetanesulfonate, 1 μM. for 3 h at 37°C. Cytotoxicity was assessed by the trypan blue test and genotoxicity was evaluated by the single cell gel (comet) assay. The results showed that exposure to 2.5% and 5% NaOCl and 8.5% citric acid resulted in a significant cytotoxic effect. NaOCl, EDTA and citric acid did not produce genotoxic effects with respect to the comet assay data for all evaluated concentrations. Although MTAD was not a cytotoxic agent, it showed significant genotoxic effects at all tested concentrations (ANOVA and Tukey's test; p<0.05). NaOCl, EDTA and citric acid were found to be cytotoxic in a dose-dependent manner, but they were not genotoxic. MTAD did not cause cell death, but presented genotoxic effects. (AU)

• Processo FAPESP: 07/01228-4 - Carcinogênese bucal quimicamente induzida pela 4-nitroquinolina 1-óxido em ratos: possíveis biomarcadores envolvidos em sua patogênese
• Beneficiário: Daniel Araki Ribeiro
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores