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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vitro genotoxicity and cytotoxicity in murine fibroblasts exposed to EDTA, NaOCl, MTAD and citric acid

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Author(s):
Juliana Soares Roter Marins [1] ; Luciana Moura Sassone [2] ; Sandra Rivera Fidel [3] ; Daniel Araki Ribeiro [4]
Total Authors: 4
Affiliation:
[1] State University of Rio de Janeiro. Dental School. Proclin Department - Brasil
[2] State University of Rio de Janeiro. Dental School. Proclin Department - Brasil
[3] State University of Rio de Janeiro. Dental School. Proclin Department - Brasil
[4] Federal University of São Paulo. Dental School. Department of Bioscience - Brasil
Total Affiliations: 4
Document type: Journal article
Source: Brazilian Dental Journal; v. 23, n. 5, p. 527-533, 2012-10-00.
Abstract

The aim of the present study was to evaluate the capacity of some root canal irrigants to induce genetic damage and/or cellular death in vitro. Murine fibroblast cells were exposed to ethylenediaminetetraacetic acid (EDTA), sodium hypochlorite (NaOCl), MTAD™ and citric acid in increasing concentrations for 3 h at 37ºC. The negative control group was treated with vehicle control (phosphate buffer solution - PBS) for 3 h at 37°C, and the positive control group was treated with methylmetanesulfonate, 1 μM. for 3 h at 37°C. Cytotoxicity was assessed by the trypan blue test and genotoxicity was evaluated by the single cell gel (comet) assay. The results showed that exposure to 2.5% and 5% NaOCl and 8.5% citric acid resulted in a significant cytotoxic effect. NaOCl, EDTA and citric acid did not produce genotoxic effects with respect to the comet assay data for all evaluated concentrations. Although MTAD was not a cytotoxic agent, it showed significant genotoxic effects at all tested concentrations (ANOVA and Tukey's test; p<0.05). NaOCl, EDTA and citric acid were found to be cytotoxic in a dose-dependent manner, but they were not genotoxic. MTAD did not cause cell death, but presented genotoxic effects. (AU)

FAPESP's process: 07/01228-4 - Medium-term oral carcinogenesis assay induced by 4-nitroquinoline 1-oxide in rats: putative biomarkers involved into its pathogenesis
Grantee:Daniel Araki Ribeiro
Support Opportunities: Research Grants - Young Investigators Grants