Alternative Low-Cost Additives to Improve the Saccharification of Lignocellulosic Biomass

Autor(es)

Brondi, Mariana G. ^{[1, 2]} ; Vasconcellos, Vanessa M. ^{[1, 2]} ; Giordano, Roberto C. ^[1] ; Farinas, Cristiane S. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Grad Program Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

^[2] EMBRAPA Instrumentacao, Rua XV Novembro 1452, BR-13560970 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Biochemistry and Biotechnology; v. 187, n. 2, p. 461-473, FEB 2019.
• Citações Web of Science: 4

Resumo

A potential strategy to mitigate problems related to unproductive adsorption of enzymes onto lignin during the saccharification of lignocellulosic biomass is the addition of lignin-blocking agents to the hydrolysis reaction medium. However, there is a clear need to find more cost-effective additives for use in large-scale processes. Here, selected alternative low-cost additives were evaluated in the saccharification of steam-exploded sugarcane bagasse using a commercial enzymatic cocktail. The addition of soybean protein, tryptone, peptone, and maize zein had positive effects on glucose release during the hydrolysis, with gains of up to 36% when 8% (w/w) soybean protein was used. These improvements were superior to those obtained using bovine serum albumin (BSA), a much more expensive protein that has been widely reported for such an application. Moreover, addition of soybean protein led to a saving of 48h in the hydrolysis, corresponding to a 66% decrease in the reactor operation time required. In order to achieve the same hydrolysis yield without the soybean additive, the enzyme loading would need to be increased by 50%. FTIR spectroscopy and nitrogen elemental analysis revealed that the additives probably acted to reduce unproductive binding of cellulolytic enzymes onto the lignin portion of the sugarcane bagasse. (AU)

• Processo FAPESP: 17/13931-3 - Proteína de soja como aditivo na hidrólise enzimática da biomassa vegetal: desenvolvimento e análise tecnoeconômica do processo
• Beneficiário: Mariana Govoni Brondi Wolf
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 14/19000-3 - Simplificação do processo de conversão de biomassa a etanol por meio da integração da produção de enzimas in-house
• Beneficiário: Cristiane Sanchez Farinas
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular