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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Alternative Low-Cost Additives to Improve the Saccharification of Lignocellulosic Biomass

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Autor(es):
Brondi, Mariana G. [1, 2] ; Vasconcellos, Vanessa M. [1, 2] ; Giordano, Roberto C. [1] ; Farinas, Cristiane S. [1, 2]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos, Grad Program Chem Engn, BR-13565905 Sao Carlos, SP - Brazil
[2] EMBRAPA Instrumentacao, Rua XV Novembro 1452, BR-13560970 Sao Carlos, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Applied Biochemistry and Biotechnology; v. 187, n. 2, p. 461-473, FEB 2019.
Citações Web of Science: 4
Resumo

A potential strategy to mitigate problems related to unproductive adsorption of enzymes onto lignin during the saccharification of lignocellulosic biomass is the addition of lignin-blocking agents to the hydrolysis reaction medium. However, there is a clear need to find more cost-effective additives for use in large-scale processes. Here, selected alternative low-cost additives were evaluated in the saccharification of steam-exploded sugarcane bagasse using a commercial enzymatic cocktail. The addition of soybean protein, tryptone, peptone, and maize zein had positive effects on glucose release during the hydrolysis, with gains of up to 36% when 8% (w/w) soybean protein was used. These improvements were superior to those obtained using bovine serum albumin (BSA), a much more expensive protein that has been widely reported for such an application. Moreover, addition of soybean protein led to a saving of 48h in the hydrolysis, corresponding to a 66% decrease in the reactor operation time required. In order to achieve the same hydrolysis yield without the soybean additive, the enzyme loading would need to be increased by 50%. FTIR spectroscopy and nitrogen elemental analysis revealed that the additives probably acted to reduce unproductive binding of cellulolytic enzymes onto the lignin portion of the sugarcane bagasse. (AU)

Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
Beneficiário:Roberto de Campos Giordano
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático
Processo FAPESP: 14/19000-3 - Simplificação do processo de conversão de biomassa a etanol por meio da integração da produção de enzimas in-house
Beneficiário:Cristiane Sanchez Farinas
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Regular
Processo FAPESP: 17/13931-3 - Proteína de soja como aditivo na hidrólise enzimática da biomassa vegetal: desenvolvimento e análise tecnoeconômica do processo
Beneficiário:Mariana Govoni Brondi
Linha de fomento: Bolsas no Brasil - Mestrado