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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Evaluation of cellulose nanocrystal addition on morphology, compression modulus and cytotoxicity of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) scaffolds

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Autor(es):
do Amaral Montanheiro, Thais Larissa [1, 2] ; Montagna, Larissa Stieven [2] ; Patrulea, Viorica [3] ; Jordan, Olivier [3] ; Borchard, Gerrit [3] ; Monteiro Lobato, Gabriela Matheus [4] ; Catalani, Luiz Henrique [4] ; Lemes, Ana Paula [2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Technol Inst Aeronaut, Div Fundamental Sci, Lab Nanotechnol, Praca Marechal Eduardo Gomes, 50 Vila Acacias, BR-12228900 Sao Jose Dos Campos, SP - Brazil
[2] Univ Fed Sao Paulo, Technol Lab Polymers & Biopolymers, 330 Vila Nair, BR-12231280 Sao Jose Dos Campos, SP - Brazil
[3] Univ Lausanne, Univ Geneva, Sch Pharmaceut Sci, 1 Rue Michel Servet, CH-1211 Geneva - Switzerland
[4] Univ Sao Paulo, Inst Chem, Lab Polymer Biomat, Ave Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Journal of Materials Science; v. 54, n. 9, p. 7198-7210, MAY 2019.
Citações Web of Science: 0
Resumo

Nanocomposite scaffolds of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with 1, 2 and 3% (wt) of cellulose nanocrystals (CNC) were produced by thermally induced phase separation. CNC presented an average length of 91 +/- 26 nm and average diameter of 7 +/- 1 nm, determined by atomic force microscopy (AFM). Field emission gun scanning electron microscopy (FEG-SEM) and X-ray microtomography showed porous morphology with interconnected pores, porosity between 41 and 77% and micron-sized CNC dispersion along the samples. Pore distribution after introducing CNC was less regular with an average reduction of 37% in the porosity. The compression modulus was improved about 28% for PHBV/1% CNC, 25% for PHBV/2% CNC and 63% for PHBV/3% CNC. Mouse fibroblasts attached and proliferated better on PHBV/CNC scaffolds surface than on neat PHBV or tissue culture plate controls. After 10 days of cell culture, PHBV/2% CNC sample enhanced cell proliferation with 42%, compared to neat PHBV. Therefore, the addition of CNC can improve both compressive modulus and cell proliferation, making the composite scaffold a potential candidate for tissue engineering. (AU)

Processo FAPESP: 13/27064-9 - Utilização de nanocristais de celulose na produção de nanocompósitos poliméricos para aplicações na área médica
Beneficiário:Ana Paula Lemes
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/21442-6 - Polímeros sintéticos e naturais aplicados à engenharia de tecido
Beneficiário:Luiz Henrique Catalani
Linha de fomento: Auxílio à Pesquisa - Temático