Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Structural characterization of cationic DODAB bilayers containing C24:1 beta-glucosylceramide

Texto completo
Autor(es):
Martins, Leticia S. [1] ; Nomura, Daniela A. [2] ; Duarte, Evandro L. [2] ; Riske, Karin A. [1] ; Teresa Lamy, M. [2] ; Rozenfeld, Julio H. K. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo, Dept Biofis, Escola Paulista Med, R Botucatu 862, BR-04023062 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Fis, CP 66318, BR-05315970 Sao Paulo, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES; v. 1861, n. 3, p. 643-650, MAR 1 2019.
Citações Web of Science: 0
Resumo

The effect of 5 mol%, 9 mol%, and 16 mol% of C24:1 beta-glucosylceramide (beta GlcCer) on the structure of cationic DODAB bilayers was investigated by means of differential scanning calorimetry (DSC), electron spin resonance (ESR) spectroscopy and fluorescence microscopy. beta GlcCer is completely miscible with DODAB at all fractions tested, since no domains were observed in fluorescence microscopy or ESR spectra. The latter showed that beta GlcCer destabilized the gel phase of DODAB bilayers by decreasing the gel phase packing. As a consequence, beta GlcCer induced a decrease in the phase transition temperature and cooperativity of DODAB bilayers, as seen in DSC thermograms. ESR spectra also showed that beta GlcCer induced an increase in DODAB fluid phase order and/or rigidity. Despite their different structures, a similar effect of loosening the gel phase packing and turning the fluid phase more rigid/organized has also been observed when low molar fractions of cholesterol were incorporated in DODAB bilayers. The structural characterization of mixed membranes made of cationic lipids and glucosylceramides may be important for developing novel immunotherapeutic tools such as vaccine adjuvants. (AU)

Processo FAPESP: 16/13368-4 - Sistemas nanoestruturados: de modelos biomiméticos de membranas a carreadores de bioativos
Beneficiário:Karin Do Amaral Riske
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 14/50983-3 - INCT 2014: fluidos complexos
Beneficiário:Antonio Martins Figueiredo Neto
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 16/19077-1 - Desenvolvimento de novos adjuvantes de vacinas capazes de ativar células T CD1d-restritas
Beneficiário:Julio Henrique Kravcuks Rozenfeld
Linha de fomento: Auxílio à Pesquisa - Regular