Structural characterization of cationic DODAB bilayers containing C24:1 beta-glucosylceramide

Autor(es)

Martins, Leticia S. ^[1] ; Nomura, Daniela A. ^[2] ; Duarte, Evandro L. ^[2] ; Riske, Karin A. ^[1] ; Teresa Lamy, M. ^[2] ; Rozenfeld, Julio H. K. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Biofis, Escola Paulista Med, R Botucatu 862, BR-04023062 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Fis, CP 66318, BR-05315970 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES; v. 1861, n. 3, p. 643-650, MAR 1 2019.
• Citações Web of Science: 0

Resumo

The effect of 5 mol%, 9 mol%, and 16 mol% of C24:1 beta-glucosylceramide (beta GlcCer) on the structure of cationic DODAB bilayers was investigated by means of differential scanning calorimetry (DSC), electron spin resonance (ESR) spectroscopy and fluorescence microscopy. beta GlcCer is completely miscible with DODAB at all fractions tested, since no domains were observed in fluorescence microscopy or ESR spectra. The latter showed that beta GlcCer destabilized the gel phase of DODAB bilayers by decreasing the gel phase packing. As a consequence, beta GlcCer induced a decrease in the phase transition temperature and cooperativity of DODAB bilayers, as seen in DSC thermograms. ESR spectra also showed that beta GlcCer induced an increase in DODAB fluid phase order and/or rigidity. Despite their different structures, a similar effect of loosening the gel phase packing and turning the fluid phase more rigid/organized has also been observed when low molar fractions of cholesterol were incorporated in DODAB bilayers. The structural characterization of mixed membranes made of cationic lipids and glucosylceramides may be important for developing novel immunotherapeutic tools such as vaccine adjuvants. (AU)

• Processo FAPESP: 16/13368-4 - Sistemas nanoestruturados: de modelos biomiméticos de membranas a carreadores de bioativos
• Beneficiário: Karin Do Amaral Riske
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/50983-3 - INCT 2014: fluidos complexos
• Beneficiário: Antonio Martins Figueiredo Neto
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/19077-1 - Desenvolvimento de novos adjuvantes de vacinas capazes de ativar células T CD1d-restritas
• Beneficiário: Julio Henrique Kravcuks Rozenfeld
• Linha de fomento: Auxílio à Pesquisa - Regular