| Full text | |
| Author(s): |
Martins, Leticia S.
[1]
;
Nomura, Daniela A.
[2]
;
Duarte, Evandro L.
[2]
;
Riske, Karin A.
[1]
;
Teresa Lamy, M.
[2]
;
Rozenfeld, Julio H. K.
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Biofis, Escola Paulista Med, R Botucatu 862, BR-04023062 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Fis, CP 66318, BR-05315970 Sao Paulo, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES; v. 1861, n. 3, p. 643-650, MAR 1 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
The effect of 5 mol%, 9 mol%, and 16 mol% of C24:1 beta-glucosylceramide (beta GlcCer) on the structure of cationic DODAB bilayers was investigated by means of differential scanning calorimetry (DSC), electron spin resonance (ESR) spectroscopy and fluorescence microscopy. beta GlcCer is completely miscible with DODAB at all fractions tested, since no domains were observed in fluorescence microscopy or ESR spectra. The latter showed that beta GlcCer destabilized the gel phase of DODAB bilayers by decreasing the gel phase packing. As a consequence, beta GlcCer induced a decrease in the phase transition temperature and cooperativity of DODAB bilayers, as seen in DSC thermograms. ESR spectra also showed that beta GlcCer induced an increase in DODAB fluid phase order and/or rigidity. Despite their different structures, a similar effect of loosening the gel phase packing and turning the fluid phase more rigid/organized has also been observed when low molar fractions of cholesterol were incorporated in DODAB bilayers. The structural characterization of mixed membranes made of cationic lipids and glucosylceramides may be important for developing novel immunotherapeutic tools such as vaccine adjuvants. (AU) | |
| FAPESP's process: | 16/13368-4 - Nanostructured systems: from membrane biomimetic models to carriers of bioactives |
| Grantee: | Karin Do Amaral Riske |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 14/50983-3 - INCT 2014: complex fluids |
| Grantee: | Antonio Martins Figueiredo Neto |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 16/19077-1 - Development of novel vaccine adjuvants capable of activating CD1d-restricted T cells |
| Grantee: | Julio Henrique Kravcuks Rozenfeld |
| Support Opportunities: | Regular Research Grants |