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Supramolecular assemblies of cationic lipid, antibiotics and polymers: preparation, characterization and activity against multiresistant bacteria and fast-growing mycobacteria

Grant number: 12/24534-1
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2013
Effective date (End): June 30, 2016
Field of knowledge:Biological Sciences - Biophysics - Molecular Biophysics
Principal Investigator:Ana Maria Carmona-Ribeiro
Grantee:Letícia Dias de Melo Carrasco
Home Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The cationic lipid dioctadecyldimethylammonium bromide (DODAB) self-assembles as bilayer fragments (BF) or large bilayer vesicles (LV) in water depending on the dispersion method and are established as potent antimicrobial agents. On the other hand, vesicles and liposomes can be used as carriers for antibiotics able to deliver drugs inside cells such as the macrophages. Novel hybrid microbicidal materials are being developed by our group based on cationic lipid and polymers to address the problem of multiresistant bacteria and /or fast-growing mycobacteria. The novel supramolecular assemblies will be characterized by dynamic light scattering, determination of antibiotic incorporation from dialysis experiments, determination of bacteria viability by plating and CFU (colony forming unities) counting and determination of macrophages viability in the presence of bacteria and the novel formulations. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
Researchers test nanoparticles against multidrug-resistant bacteria 
Researchers test nanoparticles against multidrug-resistant bacteria 

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SANCHES, LUCCAS MISSFELDT; SIQUEIRA PETRI, DENISE FREITAS; DE MELO CARRASCO, LETICIA DIAS; CARMONA-RIBEIRO, ANA MARIA. The antimicrobial activity of free and immobilized poly (diallyldimethylammonium) chloride in nanoparticles of poly (methylmethacrylate). JOURNAL OF NANOBIOTECHNOLOGY, v. 13, SEP 24 2015. Web of Science Citations: 15.
DE MELO CARRASCO, LETICIA DIAS; MELLO SAMPAIO, JORGE LUIZ; CARMONA-RIBEIRO, ANA MARIA. Supramolecular Cationic Assemblies against Multidrug-Resistant Microorganisms: Activity and Mechanism of Action. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 16, n. 3, p. 6337-6352, 2015. Web of Science Citations: 10.
CARMONA-RIBEIRO, ANA MARIA; DE MELO CARRASCO, LETICIA DIAS. Novel Formulations for Antimicrobial Peptides. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 15, n. 10, p. 18040-18083, OCT 2014. Web of Science Citations: 56.
RAGIOTO, DANIELLE A. M. T.; CARRASCO, LETICIA D. M.; CARMONA-RIBEIRO, ANA M. Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 9, p. 3183-3192, 2014. Web of Science Citations: 11.
NAVES, ALLINY F.; PALOMBO, RENATA R.; CARRASCO, LETICIA D. M.; CARMONA-RIBEIRO, ANA M. Antimicrobial Particles from Emulsion Polymerization of Methyl Methacrylate in the Presence of Quaternary Ammonium Surfactants. Langmuir, v. 29, n. 31, p. 9677-9684, AUG 6 2013. Web of Science Citations: 21.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.