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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Identification of primary and secondary metabolites and transcriptome profile of soybean tissues during different stages of hypoxia

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Autor(es):
Coutinho, Isabel Duarte [1, 2] ; Mert Henning, Liliane Marcia [3] ; Doepp, Silas Aurelian [2] ; Nepomuceno, Alexandre [3] ; Cardoso Moraes, Larissa Alexandra [3] ; Marcolino-Gomes, Juliana [3] ; Richter, Christian [2] ; Schwalbe, Harald [2] ; Colnago, Luiz Alberto [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] EMBRAPA Instrumentacao, Rua XV Novembro 1452, BR-13560970 Sao Carlos, SP - Brazil
[2] Goethe Univ Frankfurt, Ctr Biomol Magnet Resonance, Max von Laue Str 7, D-60438 Frankfurt - Germany
[3] Embrapa Soja, Rodovia Carlos Joao Strass, BR-86001970 Londrina, Parana - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: DATA IN BRIEF; v. 21, p. 1089-1100, DEC 2018.
Citações Web of Science: 0
Resumo

NMR and chromatography methods combined with mass spectrometry are the most important analytical techniques employed for plant metabolomics screening. Metabolomic analysis integrated to transcriptome screening add an important extra dimension to the information flow from DNA to RNA to protein. The most useful NMR experiment in metabolomics analysis is the proton spectra due the high receptivity of H-1 and important structural information, through proton-proton scalar coupling. Routinely, databases have been used in identification of primary metabolites, however, there is currently no comparable data for identification of secondary metabolites, mainly, due to signal overlap in normal H-1 NMR spectra and natural variation of plant. Related to spectra overlap, alternatively, better resolution can be find using H-1 pure shift and 2D NMR pulse sequence in complex samples due to spreading the resonances in a second dimension. Thus, in data brief we provide a catalogue of metabolites and expression levels of genes identified in soy leaves and roots under flooding stress. (C) 2018 The Authors. Published by Elsevier Inc. (AU)

Processo FAPESP: 15/21388-2 - Abordagens em RMN unidimensional e multidimensional para abranger o metaboloma de plantas
Beneficiário:Isabel Duarte Coutinho
Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado