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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification of primary and secondary metabolites and transcriptome profile of soybean tissues during different stages of hypoxia

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Author(s):
Coutinho, Isabel Duarte [1, 2] ; Mert Henning, Liliane Marcia [3] ; Doepp, Silas Aurelian [2] ; Nepomuceno, Alexandre [3] ; Cardoso Moraes, Larissa Alexandra [3] ; Marcolino-Gomes, Juliana [3] ; Richter, Christian [2] ; Schwalbe, Harald [2] ; Colnago, Luiz Alberto [1]
Total Authors: 9
Affiliation:
[1] EMBRAPA Instrumentacao, Rua XV Novembro 1452, BR-13560970 Sao Carlos, SP - Brazil
[2] Goethe Univ Frankfurt, Ctr Biomol Magnet Resonance, Max von Laue Str 7, D-60438 Frankfurt - Germany
[3] Embrapa Soja, Rodovia Carlos Joao Strass, BR-86001970 Londrina, Parana - Brazil
Total Affiliations: 3
Document type: Journal article
Source: DATA IN BRIEF; v. 21, p. 1089-1100, DEC 2018.
Web of Science Citations: 0
Abstract

NMR and chromatography methods combined with mass spectrometry are the most important analytical techniques employed for plant metabolomics screening. Metabolomic analysis integrated to transcriptome screening add an important extra dimension to the information flow from DNA to RNA to protein. The most useful NMR experiment in metabolomics analysis is the proton spectra due the high receptivity of H-1 and important structural information, through proton-proton scalar coupling. Routinely, databases have been used in identification of primary metabolites, however, there is currently no comparable data for identification of secondary metabolites, mainly, due to signal overlap in normal H-1 NMR spectra and natural variation of plant. Related to spectra overlap, alternatively, better resolution can be find using H-1 pure shift and 2D NMR pulse sequence in complex samples due to spreading the resonances in a second dimension. Thus, in data brief we provide a catalogue of metabolites and expression levels of genes identified in soy leaves and roots under flooding stress. (C) 2018 The Authors. Published by Elsevier Inc. (AU)

FAPESP's process: 15/21388-2 - Unidimensional and multidimensional NMR approaches to covering the plant metabolome
Grantee:Isabel Duarte Coutinho
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor