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Integrated omics approaches for the evaluation of the micromolecular phenotypic dynamics of Glycine max (SOJA) genetically modified for water stress tolerance

Grant number: 17/19702-6
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): January 01, 2018
Status:Discontinued
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal Investigator:Norberto Peporine Lopes
Grantee:Paula Carolina Pires Bueno
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated research grant:15/50488-5 - Nordeste: new science for a neglected biome, AP.SPEC
Associated scholarship(s):19/08477-7 - Integrated metabolism analysis for Glycine max responses to water stress, BE.EP.PD

Abstract

Given the rapidly rising global population and increasing demands of food production in the face of changing climates, this proposal is based on the need to promote a more in-depth understanding of the phenotypic micromolecular variation of the species that compose the current food system considering the mechanisms of adaptation to the different growth conditions. In Brazil, soybeans are the main export commodities, making the country the second world´s largest producer. However, this crop presents an impressive susceptibility to climatic and water variations, which undergoes important and undesirable losses of productivity and quality. From this perspective, the development and characterization of new genotypes more tolerant to such events corroborates to the need of increasing productivity and reducing economic losses due to this susceptibility. Therefore, because of the significant economic potential of soybeans both in Brazil and worldwide, this proposal aims to couple metabolomic and proteomic approaches as a complementary tool to the genomic and transcriptomic approaches for the evaluation and selection of molecular traits present in different genotypes of Glycine max (soybean) subjected to different water stress, drought and flood conditions, also contributing to the development and registration of new varieties with high added technologic value. With this regard, chromatographic techniques hyphenated to mass spectrometry will be used aiming at both the identification of differentially expressed primary and secondary metabolites, as well as proteomic techniques for the selection of protein markers. The data obtained will be interpreted by multivariate analysis.