Characterization and Cytotoxicity Evaluation of a Marine Sponge Biosilica

Autor(es)

Gabbai-Armelin, P. R. ^[1] ; Kido, H. W. ^[1] ; Cruz, M. A. ^[1] ; Prado, J. P. S. ^[1] ; Avanzi, I. R. ^[1] ; Custodio, M. R. ^[2] ; Renno, A. C. M. ^[1] ; Granito, R. N. ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Paulo UNIFESP, Dept Biosci, Lab Biomat & Tissue Engn, Silva Jardim 136, BR-11015020 Santos, SP - Brazil

^[2] Univ Sao Paulo, Inst Biosci, Lab Marine Invertebrates Cell Biol, Rua Matao 101, BR-05508900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MARINE BIOTECHNOLOGY; v. 21, n. 1, p. 65-75, FEB 2019.
• Citações Web of Science: 1

Resumo

Bone fractures characterize an important event in the medical healthcare, being related to traumas, aging, and diseases. In critical conditions, such as extensive bone loss and osteoporosis, the tissue restoration may be compromised and culminate in a non-union consolidation. In this context, the osteogenic properties of biomaterials with a natural origin have gained prominence. Particularly, marine sponges are promising organisms that can be exploited as biomaterials for bone grafts. Thus, the objectives of this study were to study the physicochemical and morphological properties of biosilica (BS) from sponges by using scanning electron microscopy, Fourier-transform infrared, X-ray diffraction (SEM, FTIR and XRD respectively), mineralization, and pH. In addition, tests on an osteoblast precursor cell line (MC3T3-E1) were performed to investigate its cytotoxicity and proliferation in presence of BS. Bioglass (BG) was used as gold standard material for comparison purposes. Sponge BS was obtained, and this fact was proven by SEM, FTIR, and XRD analysis. Calcium assay showed a progressive release of this ion from day 7 and a more balanced pH for BS was maintained compared to BG. Cytotoxicity assay indicated that BS had a positive influence on MC3T3-E1 cells viability and qRT-PCR showed that this material stimulated Runx2 and BMP4 gene expressions. Taken together, the results indicate a potential use of sponge biosilica for tissue engineering applications. (AU)

• Processo FAPESP: 14/23471-1 - Scaffolds de biovidro/colágeno enriquecidos com células-tronco mesenquimais: efeitos sobre o reparo de defeitos críticos em calvária de ratos
• Beneficiário: Hueliton Wilian Kido
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/20704-8 - Efeitos de compósitos de biovidro/colágeno/magnésio no processo de reparo ósseo de ratos saudáveis
• Beneficiário: Paulo Roberto Gabbai Armelin
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado