Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus)

Autor(es)

Silva Duran, Bruno Oliveira ^[1] ; Goes, Guilherme Alcaras ^[1] ; Thomazini Zanella, Bruna Tereza ^[1] ; Freire, Paula Paccielli ^[1] ; Valente, Jessica Silvino ^[1] ; Simoes Salomao, Rondinelle Artur ^[2] ; Fernandes, Ana ^[3] ; Mareco, Edson Assuncao ^[2] ; Carvalho, Robson Francisco ^[1] ; Dal-Pai-Silva, Maeli ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Morphol, Sao Paulo - Brazil

^[2] Univ West Sao Paulo UNOESTE, Sao Paulo - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Biosci, Dept Chem & Biochem, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, FEB 18 2019.
• Citações Web of Science: 2

Resumo

The postembryonic growth of skeletal muscle in teleost fish involves myoblast proliferation, migration and differentiation, encompassing the main events of embryonic myogenesis. Ascorbic acid plays important cellular and biochemical roles as an antioxidant and contributes to the proper collagen biosynthesis necessary for the structure of connective and bone tissues. However, whether ascorbic acid can directly influence the mechanisms of fish myogenesis and skeletal muscle growth remains unclear. The aim of our work was to evaluate the effects of ascorbic acid supplementation on the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus). To provide insight into the potential antioxidant role of ascorbic acid, we also treated myoblasts in vitro with menadione, which is a powerful oxidant. Our results show that ascorbic acid-supplemented myoblasts exhibit increased proliferation and migration and are protected against the oxidative stress caused by menadione. In addition, ascorbic acid increased the activity of the antioxidant enzyme superoxide dismutase and the expression of myog and mtor, which are molecular markers related to skeletal muscle myogenesis and protein synthesis, respectively. This work reveals a direct influence of ascorbic acid on the mechanisms of pacu myogenesis and highlights the potential use of ascorbic acid for stimulating fish skeletal muscle growth. (AU)

• Processo FAPESP: 16/01086-4 - Efeitos do ácido ascórbico na miogênese do músculo esquelético do pacu (Piaractus mesopotamicus)
• Beneficiário: Guilherme Alcarás de Góes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 15/03234-8 - Cultura de mioblastos do pacu (Piaractus mesopotamicus) como um modelo para entender a regulação do crescimento muscular na superordem Ostariophysi
• Beneficiário: Bruno Oliveira da Silva Duran
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/05009-4 - Análise comparativa e integrativa do transcriptoma e microRNAoma do músculo esquelético de Colossoma macropomum (tambaqui), Piaractus mesopotamicus (pacu) e seu híbrido, tambacu: abordagens moleculares, in silico, in vitro e in vivo.
• Beneficiário: Maeli Dal Pai
• Modalidade de apoio: Auxílio à Pesquisa - Regular