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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus)

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Author(s):
Silva Duran, Bruno Oliveira [1] ; Goes, Guilherme Alcaras [1] ; Thomazini Zanella, Bruna Tereza [1] ; Freire, Paula Paccielli [1] ; Valente, Jessica Silvino [1] ; Simoes Salomao, Rondinelle Artur [2] ; Fernandes, Ana [3] ; Mareco, Edson Assuncao [2] ; Carvalho, Robson Francisco [1] ; Dal-Pai-Silva, Maeli [1]
Total Authors: 10
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Morphol, Sao Paulo - Brazil
[2] Univ West Sao Paulo UNOESTE, Sao Paulo - Brazil
[3] Sao Paulo State Univ UNESP, Inst Biosci, Dept Chem & Biochem, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 9, FEB 18 2019.
Web of Science Citations: 2
Abstract

The postembryonic growth of skeletal muscle in teleost fish involves myoblast proliferation, migration and differentiation, encompassing the main events of embryonic myogenesis. Ascorbic acid plays important cellular and biochemical roles as an antioxidant and contributes to the proper collagen biosynthesis necessary for the structure of connective and bone tissues. However, whether ascorbic acid can directly influence the mechanisms of fish myogenesis and skeletal muscle growth remains unclear. The aim of our work was to evaluate the effects of ascorbic acid supplementation on the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus). To provide insight into the potential antioxidant role of ascorbic acid, we also treated myoblasts in vitro with menadione, which is a powerful oxidant. Our results show that ascorbic acid-supplemented myoblasts exhibit increased proliferation and migration and are protected against the oxidative stress caused by menadione. In addition, ascorbic acid increased the activity of the antioxidant enzyme superoxide dismutase and the expression of myog and mtor, which are molecular markers related to skeletal muscle myogenesis and protein synthesis, respectively. This work reveals a direct influence of ascorbic acid on the mechanisms of pacu myogenesis and highlights the potential use of ascorbic acid for stimulating fish skeletal muscle growth. (AU)

FAPESP's process: 16/01086-4 - Effects of ascorbic acid in the myogenesis of skeletal muscle of pacu (Piaractus mesopotamicus)
Grantee:Guilherme Alcarás de Góes
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 15/03234-8 - The pacu (Piaractus mesopotamicus) myoblast cell culture as a model to understand muscle growth regulation in Ostariophysi superorder
Grantee:Bruno Oliveira da Silva Duran
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 16/05009-4 - Comparative and integrative analysis of the transcriptome and the microRNAoma in skeletal muscle of Colossoma macropomum (tambaqui), Piaractus mesopotamicus (pacu) and its hybrid, tambacu: molecular, in silico, in vitro and in vivo approaches.
Grantee:Maeli Dal Pai
Support Opportunities: Regular Research Grants