Understanding the interactions between phosphoproteome, transcriptome and microRNAome in response to pro-growth signals in pacu (Piaractus mesopotamicus) and gilthead sea bream (Sparus aurata) myotubes.

• Grant number: 18/26428-0
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2019
• End date: April 30, 2022
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Maeli Dal Pai
• Grantee: Maeli Dal Pai
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Daniel Garcia De La Serrana Castillo ; Edson Assunção Mareco ; Josefina Blasco Mínguez

Abstract

Whole genome duplication (WGD) events are considered a major feature of the evolution of eukaryotic genomes, providing raw materials in where natural selection can act to promote increasing complexity. Two rapid rounds of WGD (known as 1R and 2R) occurred in the all vertebrates common ancestor around 550 Mya ago. A third round of WGD happened in the base of the teleost lineage (teleost-specific 3rd WGD - Ts3R), estimated to happen 350-320 million years ago (Mya). Around 15-21% of the Ts3R-derived paralogous genes have been retained due to subfunctionalization and/or neofunctionalization mechanisms. Recent studies have demonstrated differences in paralogues retention between the teleost superorders Ostariophysi and Acanthopterygii, many of them key components of the myogenesis, protein synthesis and protein degradation networks. However, despite the progresses done in the identification of these lineage-specific paralogues (LSPs), their physiological roles and regulation remain largely unknown. The pacu (Piaractus mesopotamicus) and the gilthead seabream (Sparus aurata) belong to superorders Ostariophysi and Acanthopterygii, respectively. Both species are economically relevant for the aquaculture and, therefore, objects of an intense research to improve their production.Skeletal muscle is the most abundant tissue in teleost fish, representing up to 60% of the total body mass for some species. Muscle growth is strongly dependent of the balance between protein synthesis and degradation, processes regulated by extrinsic (e.g. temperature, salinity, oxygenation) and intrinsic (e.g. transcriptional factors, hormones, miRNAs) inputs. Among them, amino acids and Igf1 have demonstrated to play an essential role in promoting protein synthesis and muscle growth.The myoblast cell culture is a very useful in vitro model to study the regulation of muscle growth. Cultured myoblasts recapitulate all the main steps during myogenesis such as lineage commitment, proliferation, fusion and myotube formation. Cell cultures can be manipulated under controlled parameters to generate experimental conditions that would allow a better understanding of muscle regulation, growth and development at different levels and under a variety of circumstances. Similarly, cell culture media can be modified to evaluate the role of nutrients or growth factors in the regulation of muscle development.In such context, the generation of high-throughput data, such as transcriptomes, microRNAomes and proteomes, have contributed enormously for the advance in the understanding of the skeletal muscle molecular biology. This approach allows for the obtaining details of the molecular networks involved in muscle physiology under distinct conditions or moments in a detail never achieved before, providing opportunities for the identification of molecular markers and new insights on the signalling pathways that regulate myogenesis.The proposed project intends to obtain the transcriptome, microRNAome and phosphoproteome of pacu and gilthead seabream myoblast cell cultures submitted to treatments with amino acids and Igf1. The integration of transcriptome, microRNAome, and phosphoproteome from these cells will provide new insights into the networks connecting pathways activation and regulation of transcription. In addition, the comparison of these global data between pacu and gilthead seabream will provide new findings on the paralogous genes, miRNAs and pathways that control myogenesis, protein synthesis and protein degradation in skeletal muscle, such as the discovery of lineage-specific markers and the better understanding of their roles in the muscle. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DURAN, BRUNO OLIVEIRA SILVA; ZANELLA, BRUNA TEREZA THOMAZINI; PEREZ, ERIKA STEFANI; MARECO, EDSON ASSUNCAO; BLASCO, JOSEFINA; DAL-PAI-SILVA, MAELI; GARCIA DE LA SERRANA, DANIEL. mino Acids and IGF1 Regulation of Fish Muscle Growth Revealed by Transcriptome and microRNAome Integrative Analyses of Pacu (Piaractus mesopotamicus) Myotube. NTERNATIONAL JOURNAL OF MOLECULAR SCIENCE, v. 23, n. 3, FEB 2022. (18/24575-6, 18/26428-0)

PEREZ, ERIKA STEFANI; DURAN, BRUNO OLIVEIRA SILVA; ZANELLA, BRUNA TEREZA THOMAZINI; DAL-PAI-SILVA, MAELI. Review: Understanding fish muscle biology in the indeterminate growth species pacu (Piaractus mesopotamicus). COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 285, p. 11-pg., 2023-11-01. (18/26428-0, 22/15932-5, 21/13905-8)

PEREZ, ERIKA STEFANI; CURY, SARAH SANTILONI; ZANELLA, BRUNA TEREZA THOMAZINI; CARVALHO, ROBSON FRANCISCO; DURAN, BRUNO OLIVEIRA SILVA; DAL-PAI-SILVA, MAELI. Identification of Novel Genes Associated with Fish Skeletal Muscle Adaptation during Fasting and Refeeding Based on a Meta-Analysis. GENES, v. 13, n. 12, p. 16-pg., 2022-12-01. (18/26428-0)

PEREZ, ERIKA S.; RIBEIRO, RAFAELA A.; ZANELLA, BRUNA T. T.; ALMEIDA, FERNANDA L. A.; BLASCO, JOSEFINA; DE LA SERRANA, DANIEL GARCIA; DAL-PAI-SILVA, MAELI; DURAN, BRUNO O. S.. Proteome of amino acids or IGF1-stimulated pacu muscle cells offers molecular insights and suggests FN1B and EIF3C as candidate markers of fish muscle growth. Biochemical and Biophysical Research Communications, v. 757, p. 15-pg., 2025-04-09. (18/26428-0, 18/24575-6)

DURAN, BRUNO OLIVEIRA SILVA; GARCIA DE LA SERRANA, DANIEL; ZANELLA, BRUNA TEREZA THOMAZINI; PEREZ, ERIKA STEFANI; MARECO, EDSON ASSUNCAO; SANTOS, VANDER BRUNO; CARVALHO, ROBSON FRANCISCO; DAL-PAI-SILVA, MAELI. An insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth. PLoS One, v. 16, n. 7, JUL 22 2021. (18/24575-6, 18/26428-0, 15/03234-8)