Tomato floral induction and flower development are orchestrated by the interplay between gibberellin and two unrelated microRNA-controlled modules

Autor(es):Mostrar menos -

Silva, Geraldo F. F. ^[1] ; Silva, Eder M. ^[1] ; Correa, Joao P. O. ^[1] ; Vicente, Mateus H. ^[2] ; Jiang, Nan ^[3] ; Notini, Marcela M. ^[1] ; Junior, Airton C. ^[1] ; De Jesus, Frederico A. ^[2] ; Castilho, Pollyanna ^[1] ; Carrera, Esther ^[4] ; Lopez-Diaz, Isabel ^[4] ; Grotewold, Erich ^[3] ; Peres, Lazaro E. P. ^[2] ; Nogueira, Fabio T. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz ESALQ, Lab Mol Genet Plant Dev, BR-13418900 Piracicaba, SP - Brazil

^[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz ESALQ, Lab Hormonal Control Plant Dev, BR-13418900 Piracicaba, SP - Brazil

^[3] Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 - USA

^[4] Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, Ingeniero Fausto Elio S-N, E-46022 Valencia - Spain

• Tipo de documento: Artigo Científico
• Fonte: NEW PHYTOLOGIST; v. 221, n. 3, p. 1328-1344, FEB 2019.
• Citações Web of Science: 8

Resumo

Age-regulated microRNA156 (miR156) and targets similarly control the competence to flower in diverse species. By contrast, the diterpene hormone gibberellin (GA) and the microRNA319-regulated TEOSINTE BRANCHED/CYCLOIDEA/PCF (TCP) transcription factors promote flowering in the facultative long-day Arabidopsis thaliana, but suppress it in the day-neutral tomato (Solanum lycopersicum). We combined genetic and molecular studies and described a new interplay between GA and two unrelated miRNA-associated pathways that modulates tomato transition to flowering. Tomato PROCERA/DELLA activity is required to promote flowering along with the miR156-targeted SQUAMOSA PROMOTER BINDING-LIKE (SPL/SBP) transcription factors by activating SINGLE FLOWER TRUSS (SFT) in the leaves and the MADS-Boxgene APETALA1(AP1)/MC at the shoot apex. Conversely, miR319-targeted LANCEOLATE represses floral transition by increasing GA concentrations and inactivating SFT in the leaves and AP1/MC at the shoot apex. Importantly, the combination of high GA concentrations/responses with the loss of SPL/SPB function impaired canonical meristem maturation and flower initiation in tomato. Our results reveal a cooperative regulation of tomato floral induction and flower development, integrating age cues (miR156 module) with GA responses and miR319-controlled pathways. Importantly, this study contributes to elucidate the mechanisms underlying the effects of GA in controlling flowering time in a day-neutral species. (AU)

• Processo FAPESP: 13/16949-0 - Regulação da arquitetura vegetativa de tomateiro (Solanum lycopersicum L.) pela via miR156-SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL)
• Beneficiário: Geraldo Felipe Ferreira e Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/23826-7 - A relação entre as vias GA (giberelina) e AGE (miR156 node) no controle do florescimento em tomateiro
• Beneficiário: Geraldo Felipe Ferreira e Silva
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 15/17892-7 - Estudo funcional de vias reguladas pelos microRNAs miR159 e miR319 durante o desenvolvimento de flores e frutos de tomateiro
• Beneficiário: Fabio Tebaldi Silveira Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Regular