Influence of follicle size on bovine oocyte lipid composition, follicular metabolic and stress markers, embryo development and blastocyst lipid content

Autor(es):Mostrar menos -

Annes, Kelly ^{[1, 2]} ; Mueller, Diego B. ^[2] ; Vilela, Jorge A. P. ^[2] ; Valente, Roniele S. ^[2] ; Caetano, Diana P. ^[2] ; Cibin, Francielli W. S. ^[2] ; Milazzotto, Marcella P. ^[1] ; Mesquita, Fernando S. ^[2] ; Belaz, Katia R. A. ^{[3, 4]} ; Eberlin, Marcos N. ^[3] ; Sudano, Mateus J. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Fed Univ ABC, Ctr Nat & Human Sci, Av Estados 5001, BR-09210580 Santo Andre, SP - Brazil

^[2] Fed Univ Pampa, Sch Vet Med, BR 472 KM 592, POB 118, BR-97501970 Uruguaiana, RS - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Inst Chem, ThoMSon Mass Spectrometry Lab, POB 6154, BR-13083970 Campinas, SP - Brazil

^[4] Fed Univ Uberlandia UFU, Inst Chem, BR-38408144 Uberlandia, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTION FERTILITY AND DEVELOPMENT; v. 31, n. 3, p. 462-472, FEB 2019.
• Citações Web of Science: 2

Resumo

This study assessed the lipid composition of oocytes from different follicle sizes and compared the expression of lipid-related genes and follicular fluid (FF) molecules between groups. We also investigated the functional consequences of differences on embryo development and blastocyst lipid deposits. Oocytes and FF were recovered from different follicle sizes. Oocytes from small (5mm) and large (6mm) bovine follicles were used to produce Day 7 expanded blastocysts (Day7Ex) and blastocysts that only became expanded at Day 8 (Day8Ex) after insemination. Oocytes from >8mm follicles had the highest lipid content. Few oocyte phospholipid variations were identified between groups. Very long chain fatty acid elongase 6 (ELOVL6) mRNA abundance was reduced in larger follicle-derived oocytes compared with the 2mm group. Increased levels of glucose, reactive oxygen species, glutathione and superoxide dismutase activity were also identified in FF from larger follicles. Large follicle-derived embryo development and lipid content of Day7Ex were greater than those derived from small follicles. Day8Ex had greater lipid deposition than Day7Ex. Oocytes and blastocysts exhibited follicle size-specific lipids. Large-follicle oocytes had increased lipid content and became Day7Ex with greater lipid deposition whereas delayed blastocoel expansion associated with a prolonged period of culture determined the lipid accumulation of Day8Ex. The FF microenvironment of large follicles seems to favour embryo development. (AU)

• Processo FAPESP: 16/05986-0 - Papel do colesterol na criotolerância e viabilidade de embriões bovinos produzidos in vitro com diferentes cinéticas de desenvolvimento.
• Beneficiário: Kelly Annes
• Modalidade de apoio: Bolsas no Brasil - Doutorado