Synergy in the interaction of amoxicillin and methylene blue with dipalmitoyl phosphatidyl choline (DPPC) monolayers

Autor(es)

Maximino, Mateus D. ^[1] ; Constantino, Carlos J. L. ^[1] ; Oliveira, Jr., Osvaldo N. ^[2] ; Alessio, Priscila ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Technol & Appl Sci, BR-19060080 Presidente Prudente, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Phys, CP 369, BR-13560970 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Surface Science; v. 476, p. 493-500, MAY 15 2019.
• Citações Web of Science: 1

Resumo

Understanding molecular-level mechanisms in the action of emergent pollutants is essential to correlate with their possible impact on living organisms in the environment and on human health. In this study, we investigate the interactions between two widely used compounds classified as emerging pollutants and Langmuir monolayers of 1,2-dipalmitoyl-sn-glycerol-3-phosphocholine (DPPC) that represent a simplified model of the lipidic structure of cell membranes. The pollutants studied were the antibiotic amoxicillin (AMX) and methylene blue (MB), a pharmaceutical drug also used as a dye in industry. AMX and MB were found to expand the surface pressure isotherms of DPPC, also affecting its morphology according to Brewster angle microscopy images. Significantly, when these compounds were mixed (MIX), monolayer expansion increased. The synergy in MIX activity was confirmed with in-situ polarization-modulated infrared reflection absorption spectroscopy (PMIRRAS) measurements, which indicated that electrostatic interaction with DPPC head groups was considerably stronger than for either AMX or MB. An adduct formed between AMX and MB in MIX also caused the DPPC monolayer thickness to increase, as inferred from measurements with grazing-incidence X-ray scattering out of the specular plane (GIXOS), in contrast to the decreased thickness induced by AMX or MB. That a mixture potentiates the interaction between contaminants and cell membrane models may be relevant for cocktail effects on living organisms. (AU)

• Processo FAPESP: 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/06534-8 - O Impacto de Poluentes Emergentes na Saúde: um Estudo em Membranas Biomiméticas
• Beneficiário: Priscila Alessio Constantino
• Modalidade de apoio: Auxílio à Pesquisa - Regular