| Texto completo | |
| Autor(es): |
Costa-Silva, T. A.
[1]
;
Camacho-Cordova, D. I.
[1]
;
Agamez-Montalvo, G. S.
[2]
;
Parizotto, L. A.
[3]
;
Sanchez-Moguel, I.
[1]
;
Pessoa-, Jr., A.
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Pharmaceut Sci, Ave Prof Lineu Prestes 580, B16 Cidade Univ, BR-05508000 Sao Paulo - Brazil
[2] Univ Fed Ceara, Fortaleza, Ceara - Brazil
[3] Univ Sao Paulo, Polytech Sch, Sao Paulo - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY; v. 49, n. 1, p. 95-104, JAN 2 2019. |
| Citações Web of Science: | 0 |
| Resumo | |
L-Asparaginase amidohydrolase (EC 3.5.1.1) has received significant attention owing to its clinical use in acute lymphoblastic leukemia treatment and non-clinical applications in the food industry to reduce acrylamide (toxic compound) formation during the frying of starchy foods. In this study, a sequential optimization strategy was used to determine the best culture conditions for L-asparaginase production from filamentous fungus Aspergillus terreus CCT 7693 by submerged fermentation. The cultural conditions were studied using a 3-level, central composite design of response surface methodology, and biomass and enzyme production were optimized separately. The highest amount of biomass (22.0 g center dot L-1) was obtained with modified Czapek-Dox medium containing glucose (14 g center dot L-1), L-proline (10 g center dot L-1), and ammonium nitrate (2 g center dot L-1) fermented at 37.2 degrees C and pH 8.56; for maximum enzyme production (13.50 U center dot g(-1)), the best condition was modified Czapek-Dox medium containing glucose (2 g center dot L-1), L-proline (10 g center dot L-1), and inoculum concentration of 4.8 x 10(8) espore center dot mL(-1) adjusted to pH 9.49 at 34.6 degrees C. The L-asparaginase production profile was studied in a 7 L bench-scale bioreactor and a final specific activity of 13.81 U center dot g(-1) was achieved, which represents an increase of 200% in relation to the initial non-optimized conditions. (AU) | |
| Processo FAPESP: | 13/08617-7 - Produção de L-Asparaginase extracelular: da bioprospecção à engenharia de um biofármaco antileucêmico |
| Beneficiário: | Adalberto Pessoa Junior |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 13/19584-2 - Produção de L-asparaginase extracelular pelo fungo Arpergillus terreus |
| Beneficiário: | Tales Alexandre da Costa e Silva |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |