Prolonged erythrocyte auto-incubation as an alternative model for oxidant generation system

Autor(es)

Humberto da Silva, Danilo Grunig ^[1] ; Chaves, Nayara Alves ^[1] ; Miyamoto, Sayuri ^[2] ; de Almeida, Eduardo Alves ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Inst Biociencias Letras & Ciencias Exatas, Dept Quim & Ciencias Ambientais, Sao Jose Do Rio Preto - Brazil

^[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil

^[3] Univ Reg Blumenau, Dept Ciencias Nat, Blumenau - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TOXICOLOGY IN VITRO; v. 56, p. 62-74, APR 2019.
• Citações Web of Science: 1

Resumo

This study investigated the effects of incubation period and melatonin treatment on red blood cell (RBC) metabolism in an auto-incubation model of H2O2-induced oxidative stress. The study was carried out on three healthy adult donors by incubating RBCs in their own plasma at 37 degrees C, or under the influence of 1 mM H2O2 with and without 100 mu M melatonin at different times (0, 1, 3 and 6 h). We assessed incubation period, treatment, as well as any interaction effects between these predictors on erythrocyte osmoregulation, hemolytic rate, oxidative stress markers, and adenylate nucleotide levels. We did not find any relevant effects of both incubation period and treatments on osmotic, antioxidant and adenylate parameters. On the other hand, hemolysis degree and biomolecule oxidation levels in the plasma increased over time, 3-fold and about 25%, respectively, regardless any treatment influence. H2O2 treatment more than doubled protein carbonyl groups, regardless time in plasma, and in a time-depending way in erythrocyte membrane extract, effects that were neutralized by melatonin treatment. Through multivariate analyses, we could expand the understanding of energy and redox metabolisms in the maintenance of cellular integrity and metabolic homeostasis. Another interesting observation was the 65-75% contribution of the oxidative lesion markers on hemolysis. Hence, these findings suggested a new and more intuitive RBC suspension model and reinforced the beneficial use of melatonin in human disorders. (AU)

• Processo FAPESP: 13/07937-8 - Redoxoma
• Beneficiário: Ohara Augusto
• Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 15/25983-2 - Metabolismo redox eritrocitário: implicações para o uso de melatonina no tratamento de pessoas com anemia falciforme
• Beneficiário: Danilo Grunig Humberto da Silva
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado