Genotoxic effects of photodynamic therapy in laryngeal cancer cells - An in vitro study

Autor(es)

Guedes de Oliveira Moraes, Carlos Dailton ^[1] ; Godoi, Bruno Henrique ^[1] ; Silva Carvalho, Isabel Chaves ^[1] ; Pinto, Jessica Cristina ^[1] ; Rossato, Rafaella Carvaiho ^[1] ; da Silva, Newton Soares ^[2] ; Soares, Cristina Pacheco ^[1]

Afiliação do(s) autor(es)

^[1] Univ Vale Paraiba UNIVAP, Inst Res & Dev IP&D, Lab Dynam Cellular Compartments, BR-12244000 Sao Paulo - Brazil

^[2] Univ Vale Paraiba UNIVAP, Inst Res & Dev IP&D, Lab Cell Biol & Tissue, BR-12244000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Experimental Biology and Medicine; v. 244, n. 3, p. 262-271, MAR 2019.
• Citações Web of Science: 1

Resumo

Photodynamic therapy provides the formation of reactive oxygen species that are capable of inducing cell death. Human laryngeal carcinoma (HEp-2) cells have been evaluated in this study under PDT treatment. Cells were treated with photosensitizer aluminum phthalocyanine tetrasulfonate (AlPcS4) and irradiated with a Biopdi/Irrad-Led5 660 LED with 660 nm wavelength, intensity of delivered light of 25 mW/cm(2), power of 70 mW, fluence of 5 J/cm(2) for 24 h and 48 h, and then evaluated. Cell population was not increased by PDT treatment after the tested period. The apoptosis assay demonstrated that control groups exhibit approximately 60% of living cells in the 24 h and 48 h periods, however. A significant increase in apoptotic cells was observed after the photodynamic therapy treatments, for both 24 and 48 h groups. Over 50% of cells were under apoptosis after photodynamic therapy, evidencing a death process generated from the oxidative damage of the treatment. Comet assay and micronucleus assessments, both of which evaluate genotoxicity, demonstrated favorable results to damages caused by the photodynamic therapy treatment. Thus, photodynamic therapy is proposed to damage nuclear cells and the subcellular structure of carcinogenic cells. (AU)

• Processo FAPESP: 13/20054-8 - Estudos de adesão, proliferação e de morte celular na interação de células com materiais nanoestruturados
• Beneficiário: Newton Soares da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular