Wide diversity of methane and short-chain alkane metabolisms in uncultured archaea

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Borrel, Guillaume ^[1] ; Adam, Panagiotis S. ^{[2, 1]} ; McKay, Luke J. ^[3] ; Chen, Lin-Xing ^[4] ; Sierra-Garcia, Isabel Natalia ^[5] ; Sieber, Christian M. K. ^{[4, 6]} ; Letourneur, Quentin ^[7] ; Ghozlane, Amine ^[7] ; Andersen, Gary L. ^[8] ; Li, Wen-Jun ^{[9, 10]} ; Hallam, Steven J. ^{[11, 12]} ; Muyzer, Gerard ^[13] ; de Oliveira, Valeria Maia ^[5] ; Inskeep, William P. ^[14] ; Banfield, Jillian F. ^[4] ; Gribaldo, Simonetta ^[1]

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^[1] Inst Pasteur, Dept Microbiol, Unit Evolutionary Biol Microbial Cell, Paris - France

^[2] Univ Paris Diderot, Sorbonne Paris Cite, Paris - France

^[3] Montana State Univ, Ctr Biofilm Engn, Bozeman, MT 59717 - USA

^[4] Univ Calif Berkeley, Dept Earth & Planetary Sci, Berkeley, CA 94720 - USA

^[5] Campinas Univ UNICAMP, Microbial Resources Div, Res Ctr Chem Biol & Agr, Campinas, SP - Brazil

^[6] Joint Genome Inst, Dept Energy, Walnut Creek, CA - USA

^[7] Inst Pasteur, Bioinformat & Biostat Hub, C3BI, Paris - France

^[8] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 - USA

^[9] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou, Guangdong - Peoples R China

^[10] Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Key Lab Plant Resources, Guangzhou, Guangdong - Peoples R China

^[11] Univ British Columbia, Dept Microbiol & Immunol, ECOSCOPE Training Program, Grad Program Bioinformat, Vancouver, BC - Canada

^[12] Univ British Columbia, Genome Sci & Technol Training Program, Vancouver, BC - Canada

^[13] Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Dept Freshwater & Marine Ecol, Microbial Syst Ecol, Amsterdam - Netherlands

^[14] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 - USA

• Tipo de documento: Artigo Científico
• Fonte: NATURE MICROBIOLOGY; v. 4, n. 4, p. 603-613, APR 2019.
• Citações Web of Science: 15

Resumo

Methanogenesis is an ancient metabolism of key ecological relevance, with direct impact on the evolution of Earth's climate. Recent results suggest that the diversity of methane metabolisms and their derivations have probably been vastly underestimated. Here, by probing thousands of publicly available metagenomes for homologues of methyl-coenzyme M reductase complex (MCR), we have obtained ten metagenome-assembled genomes (MAGs) belonging to potential methanogenic, anaerobic methanotrophic and short-chain alkane-oxidizing archaea. Five of these MAGs represent under-sampled (Verstraetearchaeota, Methanonatronarchaeia, ANME-1 and GoM-Arc1) or previously genomically undescribed (ANME-2c) archaeal lineages. The remaining five MAGs correspond to lineages that are only distantly related to previously known methanogens and span the entire archaeal phylogeny. Comprehensive comparative annotation substantially expands the metabolic diversity and energy conservation systems of MCR-bearing archaea. It also suggests the potential existence of a yet uncharacterized type of methanogenesis linked to short-chain alkane/fatty acid oxidation in a previously undescribed class of archaea ('Candidatus Methanoliparia'). We redefine a common core of marker genes specific to methanogenic, anaerobic methanotrophic and short-chain alkane-oxidizing archaea, and propose a possible scenario for the evolutionary and functional transitions that led to the emergence of such metabolic diversity. (AU)

• Processo FAPESP: 13/20436-8 - Investigação da degradação anaeróbia de óleo cru em microcosmos a partir da microbiota de reservatórios de petróleo brasileiros
• Beneficiário: Isabel Natalia Sierra García
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 11/14501-6 - Investigação da microbiota e dos processos envolvidos na degradação anaeróbia de petróleo em reservatórios brasileiros
• Beneficiário: Isabel Natalia Sierra García
• Modalidade de apoio: Bolsas no Brasil - Doutorado