Metabolite and transcript profiling of Guinea grass (Panicum maximum Jacq) response to elevated [CO2] and temperature

Autor(es)

Wedow, Jessica M. ^{[1, 2]} ; Yendrek, Craig R. ^{[1, 2]} ; Mello, Tathyana R. ^[3] ; Creste, Silvana ^[4] ; Martinez, Carlos A. ^[3] ; Ainsworth, Elizabeth A. ^{[1, 2, 5]}

Afiliação do(s) autor(es)

^[1] Univ Illinois, Dept Plant Biol, 1201 W Gregory Dr, 147 ERML, Urbana, IL 61801 - USA

^[2] Univ Illinois, Carl R Woese Inst Genom Biol, 1201 W Gregory Dr, 147 ERML, Urbana, IL 61801 - USA

^[3] Univ Sao Paulo, FFCLRP, Dept Biol, Ribeirao Preto, SP - Brazil

^[4] Inst Agron IAC, Ctr Cana, Ribeirao Preto - Brazil

^[5] ARS, USDA, Global Change & Photosynthesis Res Unit, Urbana, IL 61801 - USA

• Tipo de documento: Artigo Científico
• Fonte: METABOLOMICS; v. 15, n. 4 APR 2019.
• Citações Web of Science: 1

Resumo

IntroductionBy mid-century, global atmospheric carbon dioxide concentration ({[}CO2]) is predicted to reach 600molmol(-1) with global temperatures rising by 2 degrees C. Rising {[}CO2] and temperature will alter the growth and productivity of major food and forage crops across the globe. Although the impact is expected to be greatest in tropical regions, the impact of climate-change has been poorly studied in those regions.ObjectivesThis experiment aimed to understand the effects of elevated {[}CO2] (600molmol(-1)) and warming (+2 degrees C), singly and in combination, on Panicum maximum Jacq. (Guinea grass) metabolite and transcript profiles.MethodsWe created a de novo assembly of the Panicum maximum transcriptome. Leaf samples were taken at two time points in the Guinea grass growing season to analyze transcriptional and metabolite profiles in plants grown at ambient and elevated {[}CO2] and temperature, and statistical analyses were used to integrate the data.ResultsElevated temperature altered the content of amino acids and secondary metabolites. The transcriptome of Guinea grass shows a clear time point separations, with the changes in the elevated temperature and {[}CO2] combination plots.ConclusionField transcriptomics and metabolomics revealed that elevated temperature and {[}CO2] result in alterations in transcript and metabolite profiles associated with environmental response, secondary metabolism and stomatal function. These metabolic responses are consistent with greater growth and leaf area production under elevated temperature and {[}CO2]. These results show that tropical C-4 grasslands may have unpredicted responses to global climate change, and that warming during a cool growing season enhances growth and alleviates stress. (AU)

• Processo FAPESP: 08/58075-8 - Miniface climate-change impact experiment to analyze the effects of elevated CO2 and warming on photosynthesis, gene expression, biochemistry, growth, nutrient dynamics and yield of two contrasting tropical forage species
• Beneficiário: Carlos Alberto Martinez y Huaman
• Linha de fomento: Auxílio à Pesquisa - Programa de Pesquisa sobre Mudanças Climáticas Globais - Temático