Antimicrobial activity of bacteriocin-like inhibitory substance produced by Pediococcus pentosaceus: from shake flasks to bioreactor

Autor(es)

de Souza de Azevedo, Pamela Oliveira ^[1] ; Converti, Attilio ^[2] ; Gierus, Martin ^[3] ; de Souza Oliveira, Ricardo Pinheiro ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Biochem & Pharmaceut Technol, Sao Paulo, SP - Brazil

^[2] Dept Civil Chem & Environm Engn, Pole Chem Engn, Via Opera Pia 15, I-16145 Genoa - Italy

^[3] Univ Nat Resources & Life Sci BOKU, Dept Agrobiotechnol, Inst Anim Nutr Livestock Prod & Nutr Physiol TTE, IFA Tulln, Vienna - Austria

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR BIOLOGY REPORTS; v. 46, n. 1, p. 461-469, FEB 2019.
• Citações Web of Science: 1

Resumo

Bacteriocins are peptides produced by various species of bacteria, especially lactic acid bacteria (LAB), which have a large spectrum of action against spoilage bacteria and foodborne pathogens. However, when not entirely characterized, they are alternatively called bacteriocin-like inhibitory substances (BLIS). Pediococcus pentosaceus ATCC 43200 grew and produced BLIS optimally when cultivated anaerobically in bioreactor for 24h at 30 degrees C and 200rpm in De Man, Rogosa and Sharp (MRS) medium supplemented with 1.5% peptone. Under such optimal conditions, the cell mass concentration (3.41g/L) was 66% higher, the generation time (1.28h) 38% shorter and the BLIS activity against different indicator strains significantly higher than in MRS medium without any supplement taken as a control, and the exponential phase started 4h before. The agar diffusion method showed BLIS inhibition halos against LAB strains with diameter in the range 11.0-19.5mm and specific areas between 377.1 and 2654.6mm(2)/mL, while BLIS activity against Listeria strains was better quantified by the liquid medium assay that showed, for the fermented broth without any dilution, 100 and 50% inhibition of Listeria innocua and Listeria seeligeri growth, respectively. These results highlight the potential of P. pentosaceus BLIS as a natural antimicrobial for application in the food industry. (AU)

• Processo FAPESP: 16/06284-9 - Produção biotecnológica de pediocina: otimização do rendimento e efeitos biológicos sobre células do sistema imune
• Beneficiário: Ricardo Pinheiro de Souza Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular