| Texto completo | |
| Autor(es): |
de Souza de Azevedo, Pamela Oliveira
[1]
;
Converti, Attilio
[2]
;
Gierus, Martin
[3]
;
de Souza Oliveira, Ricardo Pinheiro
[1]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Dept Biochem & Pharmaceut Technol, Sao Paulo, SP - Brazil
[2] Dept Civil Chem & Environm Engn, Pole Chem Engn, Via Opera Pia 15, I-16145 Genoa - Italy
[3] Univ Nat Resources & Life Sci BOKU, Dept Agrobiotechnol, Inst Anim Nutr Livestock Prod & Nutr Physiol TTE, IFA Tulln, Vienna - Austria
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | MOLECULAR BIOLOGY REPORTS; v. 46, n. 1, p. 461-469, FEB 2019. |
| Citações Web of Science: | 1 |
| Resumo | |
Bacteriocins are peptides produced by various species of bacteria, especially lactic acid bacteria (LAB), which have a large spectrum of action against spoilage bacteria and foodborne pathogens. However, when not entirely characterized, they are alternatively called bacteriocin-like inhibitory substances (BLIS). Pediococcus pentosaceus ATCC 43200 grew and produced BLIS optimally when cultivated anaerobically in bioreactor for 24h at 30 degrees C and 200rpm in De Man, Rogosa and Sharp (MRS) medium supplemented with 1.5% peptone. Under such optimal conditions, the cell mass concentration (3.41g/L) was 66% higher, the generation time (1.28h) 38% shorter and the BLIS activity against different indicator strains significantly higher than in MRS medium without any supplement taken as a control, and the exponential phase started 4h before. The agar diffusion method showed BLIS inhibition halos against LAB strains with diameter in the range 11.0-19.5mm and specific areas between 377.1 and 2654.6mm(2)/mL, while BLIS activity against Listeria strains was better quantified by the liquid medium assay that showed, for the fermented broth without any dilution, 100 and 50% inhibition of Listeria innocua and Listeria seeligeri growth, respectively. These results highlight the potential of P. pentosaceus BLIS as a natural antimicrobial for application in the food industry. (AU) | |
| Processo FAPESP: | 16/06284-9 - Produção biotecnológica de pediocina: otimização do rendimento e efeitos biológicos sobre células do sistema imune |
| Beneficiário: | Ricardo Pinheiro de Souza Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |