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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antimicrobial activity of bacteriocin-like inhibitory substance produced by Pediococcus pentosaceus: from shake flasks to bioreactor

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Author(s):
de Souza de Azevedo, Pamela Oliveira [1] ; Converti, Attilio [2] ; Gierus, Martin [3] ; de Souza Oliveira, Ricardo Pinheiro [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Dept Biochem & Pharmaceut Technol, Sao Paulo, SP - Brazil
[2] Dept Civil Chem & Environm Engn, Pole Chem Engn, Via Opera Pia 15, I-16145 Genoa - Italy
[3] Univ Nat Resources & Life Sci BOKU, Dept Agrobiotechnol, Inst Anim Nutr Livestock Prod & Nutr Physiol TTE, IFA Tulln, Vienna - Austria
Total Affiliations: 3
Document type: Journal article
Source: MOLECULAR BIOLOGY REPORTS; v. 46, n. 1, p. 461-469, FEB 2019.
Web of Science Citations: 1
Abstract

Bacteriocins are peptides produced by various species of bacteria, especially lactic acid bacteria (LAB), which have a large spectrum of action against spoilage bacteria and foodborne pathogens. However, when not entirely characterized, they are alternatively called bacteriocin-like inhibitory substances (BLIS). Pediococcus pentosaceus ATCC 43200 grew and produced BLIS optimally when cultivated anaerobically in bioreactor for 24h at 30 degrees C and 200rpm in De Man, Rogosa and Sharp (MRS) medium supplemented with 1.5% peptone. Under such optimal conditions, the cell mass concentration (3.41g/L) was 66% higher, the generation time (1.28h) 38% shorter and the BLIS activity against different indicator strains significantly higher than in MRS medium without any supplement taken as a control, and the exponential phase started 4h before. The agar diffusion method showed BLIS inhibition halos against LAB strains with diameter in the range 11.0-19.5mm and specific areas between 377.1 and 2654.6mm(2)/mL, while BLIS activity against Listeria strains was better quantified by the liquid medium assay that showed, for the fermented broth without any dilution, 100 and 50% inhibition of Listeria innocua and Listeria seeligeri growth, respectively. These results highlight the potential of P. pentosaceus BLIS as a natural antimicrobial for application in the food industry. (AU)

FAPESP's process: 16/06284-9 - Biotechnology production of pediocin: optimization of yield and biological effects on cells of the immune system
Grantee:Ricardo Pinheiro de Souza Oliveira
Support Opportunities: Regular Research Grants