Optimization of pediocin A production in Pediococcus pentosaceus ATCC 43200

• Grant number: 15/24777-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): October 01, 2016
• Effective date (End): September 30, 2019
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Ricardo Pinheiro de Souza Oliveira
• Grantee: Taís Mayumi Kuniyoshi
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 18/02519-7 - Pediocin heterologous expression and its effects over Listeria spp. and human gut microbial population, BE.EP.PD

Abstract

A significant effort is underway to develop new antimicrobial compounds for both food and pharmaceutical industry. Bacteriocins are peptides produced by a number of bacteria that harbor bacteriostatic or bactericide effect over pathogenic and deleterious microorganism present in industrial processes. The ones produced by lactic acid bacteria (LABs) present an interest on industry as some of them are probiotic, therefore, acknowledged as safe on this field. In this context, the aim of this project is the maximization of pediocin A production by Pediococcus pentosaceus ATCC 43200 probiotic bacterium through molecular biology tools combined with techniques of scale up improvement on fermentation process. The first step of this project consists in the identification pediocin A gene from P. pentosaceus ATCC43200 and the physical chemical characterization of this bacteriocin. Secondly, the construction of a recombinant strain of P. pentosaceus ATCC 43200 over-expressing heterologously the induction factor and subsequently the comparison of its pediocin productivity with the corresponding wild type via qPCR in order to quantify pediocin transcripts. Finally, we intend to evaluate the effect of different parameters on pediocin A productivity in the recombinant strain of P. pentosaceus ATCC 43200. For this purpose, the experiments will be carry out using fractional factorial designs and/or rotational central composite design (RCCD). The results obtained will lead to establish the scale up parameters, starting from small scale assays followed by 3L discontinuous bioreactor.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PORTO, MARIA CAROLINA W.; KUNIYOSHI, TAIS MAYUMI; AZEVEDO, P. O. S.; VITOLO, MICHELE; OLIVEIRA, R. P. S.. Pediococcus spp.: An important genus of lactic acid bacteria and pediocin producers. BIOTECHNOLOGY ADVANCES, v. 35, n. 3, p. 361-374, MAY-JUN 2017. (16/06284-9, 15/24777-0)

O'CONNOR, PAULA M.; KUNIYOSHI, TAIS M.; OLIVEIRA, RICARDO P. S.; HILL, COLIN; ROSS, REYNOLDS PAUL; COTTER, PAUL D.. Antimicrobials for food and feed; a bacteriocin perspective. CURRENT OPINION IN BIOTECHNOLOGY, v. 61, p. 160-167, FEB 2020. (18/04385-8, 15/24777-0, 18/02519-7)

KUNIYOSHI, TAIS MAYUMI; NOBREGA MENDONCA, CARLOS MIGUEL; VIEIRA, VIVIANE BORGES; ROBL, DIOGO; GOMBOSSY DE MELO FRANCO, BERNADETTE DORA; TODOROV, SVETOSLAV DIMITROV; TOME, ELISABETTA; O'CONNOR, PAULA MARY; CONVERTI, ATTILIO; ARAUJO, WELINGTON LUIZ; et al. ediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagass. Bioresource Technology, v. 338, OCT 2021. (13/07914-8, 18/04385-8, 15/24777-0, 18/25511-1)

KUNIYOSHI, TAIS MAYUMI; NOBREGA MENDONCA, CARLOS MIGUEL; VIEIRA, VIVIANE BORGES; ROBL, DIOGO; GOMBOSSY DE MELO FRANCO, BERNADETTE DORA; TODOROV, SVETOSLAV DIMITROV; TOME, ELISABETTA; O'CONNOR, PAULA MARY; CONVERTI, ATTILIO; ARAUJO, WELINGTON LUIZ; et al. Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse. APPLIED GEOCHEMISTRY, v. 131, AUG 2021. (18/04385-8, 18/25511-1, 15/24777-0, 13/07914-8)