Applicability of the SNPforID 52-plex panel for human identification and ancestry evaluation in a Brazilian population sample by next-generation sequencing

Autor(es):Mostrar menos -

do Valle-Silva, Guilherme ^[1] ; Nunes de Souza, Flavia Djenane ^[1] ; Marcorin, Leticia ^[2] ; Eburneo Pereira, Alison Luis ^[2] ; Telles Carratto, Thassia Mayra ^[1] ; Debortoli, Guilherme ^{[2, 3]} ; Guimaraes de Oliveira, Maria Luiza ^[2] ; de Aguiar Fracasso, Nadia Carolina ^[2] ; de Andrade, Edilene Santos ^[4] ; Donadi, Eduardo Antonio ^[5] ; Norton, Heather L. ^[6] ; Parra, Esteban J. ^[3] ; Simoes, Aguinaldo Luiz ^[2] ; Castelli, Erick C. ^[7] ; Mendes-Junior, Celso Teixeira ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Quim, Lab Pesquisas Forenses & Genom, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Genet, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Univ Toronto Mississauga, Dept Anthropol, Mississauga, ON L5L 1C6 - Canada

^[4] Barretos Canc Hosp, Mol Oncol Res Ctr, Barretos - Brazil

^[5] Univ Sao Paulo, Div Imunol Clin, Dept Clin Med, Fac Med Ribeirao Preto, BR-14048900 Ribeirao Preto, SP - Brazil

^[6] Univ Cincinnati, Dept Anthropol, Cincinnati, OH 45221 - USA

^[7] Sao Paulo State Univ UNESP, Sch Med, Dept Pathol, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FORENSIC SCIENCE INTERNATIONAL-GENETICS; v. 40, p. 201-209, MAY 2019.
• Citações Web of Science: 1

Resumo

SNP analysis is of paramount importance in forensic genetics. The development of new technologies in next-generation sequencing allowed processing a large number of markers in various samples simultaneously. Although SNPs are less informative than STRs, they present lower mutation rates and perform better when using degraded samples. Some SNP systems were developed for forensic usage, such as the SNPforID 52-plex, from the SNPforID Consortium, containing 52 bi-allelic SNPs for human identification. In this paper we evaluated the informativeness of this system in a Brazilian population sample (n = 340). DNA libraries were prepared using a customized HaloPlex Target Enrichment System kit (Agilent Technologies, Inc.) and sequenced in the MiSeq Personal Sequencer platform (Illumina Inc.). The methodology presented here allowed the analysis of 51 out of 52 SNPforID markers. Allele frequencies and forensic parameters were estimated, revealing high informativeness: the combined match probability and power of exclusion were 6.48x10(-21) and 0.9997, respectively. Population admixture analysis indicates high European contribution (more than 70%) and low Amerindian contribution (less than 10%) in our population, while individual admixture analyses were consistent with the majority of individuals presenting high European contribution. This study demonstrates that the 52-plex kit is suitable for forensic cases in a Brazilian population, presenting results comparable with those obtained using a 16 STR panel. (AU)

• Processo FAPESP: 13/15447-0 - Sequenciamento de nova geração das regiões regulatórias e exônicas de dez genes envolvidos na biossíntese de melanina em amostra da população brasileira
• Beneficiário: Celso Teixeira Mendes Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular