Heat shock-induced apoptosis in germ line cells of Triatoma infestans Klug

Autor(es)

Maria Luiza S. Mello ^[1] ; Sílvya S. Maria ^[2] ; Maria Cristina H. Tavares

Afiliação do(s) autor(es)

^[1] Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Biologia Celular

^[2] Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Biologia Celular

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 23, n. 2, p. 301-304, 2000-06-00.

Resumo

The survival of Triatoma infestans and of somatic cells from this species is affected by heat shock. In this study, we examined the cell death responses of male germ line cells from 5th instar nymphs 7 and 30 days after heat shock exposure (40ºC, 1 h). The preparations were stained by the Feulgen reaction and the TUNEL immunocytochemical assay. Apoptosis was elicited by heat shock, with positive TUNEL responses in spermatogonial chromatin and chromosomes, spermatids and sperm cells. Spermatogonia were most affected seven days after the shock whereas some spermatids and sperm cells exhibited DNA fragmentation only thirty days after heat shock. The rate of cell death varied among the cells. In some cases, cellular differentiation was unaffected by heat shock, with DNA fragmentation occurring towards the end of spermatogenesis. (AU)

• Processo FAPESP: 99/02547-8 - Conteúdo de DNA e organização cromatínica em células em processos de transformação, tumorigênese e morte celular
• Beneficiário: Maria Luiza Silveira Mello
• Modalidade de apoio: Auxílio à Pesquisa - Regular