Multiple aPDT sessions on periodontitis in rats treated with chemotherapy: histomorphometrical, immunohistochemical, immunological and microbiological analyses

Autor(es)

Longo, Mariellen ^[1] ; Garcia, Valdir Gouveia ^[1] ; Ervolino, Edilson ^[2] ; Ferro Alves, Marcio Luiz ^[1] ; Duque, Cristiane ^[3] ; Wainwright, Mark ^[4] ; Theodoro, Leticia Helena ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Sch Dent, Dept Surg & Integrated Clin, Div Periodont, UNESP, Aracatuba, SP - Brazil

^[2] Sao Paulo State Univ, Sch Dent, Dept Basic Sci, UNESP, Aracatuba, SP - Brazil

^[3] Sao Paulo State Univ, Sch Dent, Dept Paediat & Social Dent, UNESP, Aracatuba, SP - Brazil

^[4] Liverpool John Moores Univ, Sch Pharm & Biomol Sci, Liverpool, Merseyside - England

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 25, p. 92-102, MAR 2019.
• Citações Web of Science: 2

Resumo

Background: The aim of this study was to evaluate the effect of multiple sessions of antimicrobial photodynamic therapy (aPDT) on the treatment of experimental periodontitis (EP) in rats treated with chemotherapy. Methods: Chemotherapy using 5-fluorouracil (5-FU) consisted of intraperitoneal administration of 60 and 40 mg/kg of 5-FU. 120 rats were subjected to chemotherapy with 5-FU and divided into groups: PT (periodontal treatment); PT + 1aPDT (PT and single aPDT session); PT + 4aPDT(PT and 4 sessions of aPDT); 1aPDT (single aPDT session); 4aPDT(4 sessions of aPDT). EP was induced in the mandibular molars via ligature placement. The alveolar bone loss (ABL) area in the furcation region was analysed histometrically. Proliferating cell nuclear antigen (PCNA), tartrate-resistant acid phosphatase (TRAP), receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG) and cleaved caspase-3 (CC3) were analysed by immunohistochemistry. Prostaglandin E2 was quantified using an ELISA, tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 were assessed using a multiplex method. The prevalence of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella nigrescens, Prevotella intermedia and Fusobacterium nucleatum was assessed using PCR. The data were statistically analysed (P < 0.05). Results: The PT + 4aPDT group showed lower ABL than the PT or 1aPDT groups on day 7. Rats treated with aPDT showed a higher number of PCNA-positive cells with reduced immunolabeling of RANKL. Significant reductions in Prevotella nigrescens were observed in the PT + 4aPDT group and in Aggregatibacter actinomycetemcomitans for the 1aPDT and 4aPDT groups. Conclusion: Repeated sessions of aPDT as an adjunct or alternative therapy were effective at reducing ABL, regulating bone metabolism, and reducing Prevotella nigrescens and Aggregatibacter actinomycetemcomitans. (AU)

• Processo FAPESP: 12/08649-3 - Avaliação de diferentes protocolos de aplicação da terapia fotodinâmica antimicrobiana (aPDT) no tratamento da periodontite induzida em ratos imunossuprimidos com droga quimioterápica: estudo histomorfométrico,imunoistoquímico e imunológico
• Beneficiário: Letícia Helena Theodoro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/05864-0 - AVALIAÇÃO DE DIFERENTES PROTOCOLOS DE APLICAÇÃO DA TERAPIA FOTODINÂMICA ANTIMICROBIANA (aPDT) NO TRATAMENTO DA PERIODONTITE INDUZIDA EM RATOS IMUNOSSUPRIMIDOS COM DROGA QUIMIOTERÁPICA: ESTUDO HISTOMORFOMÉTRICO, IMUNOISTOQUÍMICO E IMUNOLÓGICO.
• Beneficiário: Mariéllen Longo
• Modalidade de apoio: Bolsas no Brasil - Doutorado