Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Eicosapentaenoic Acid Reduces Adiposity, Glucose Intolerance and Increases Oxygen Consumption Independently of Uncoupling Protein 1

Texto completo
Autor(es):
Pahlavani, Mandana [1, 2] ; Ramalingam, Latha [1, 2] ; Miller, Emily K. [1, 2] ; Scoggin, Shane [1, 2] ; Menikdiwela, Kalhara R. [1, 2] ; Kalupahana, Nishan S. [1, 2, 3] ; Festuccia, William T. [4] ; Moustaid-Moussa, Naima [1, 2]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Texas Tech Univ, Dept Nutr Sci, Lubbock, TX 79409 - USA
[2] Texas Tech Univ, Obes Res Cluster, Lubbock, TX 79409 - USA
[3] Univ Peradeniya, Fac Med, Dept Physiol, Peradeniya 20400 - Sri Lanka
[4] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508 Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: MOLECULAR NUTRITION & FOOD RESEARCH; v. 63, n. 7 APR 2019.
Citações Web of Science: 3
Resumo

Scope Brown adipose tissue (BAT) dissipates energy through uncoupling protein 1 (UCP1) and has been proposed as an anti-obesity target. It was reported previously that a high-fat (HF) diet enriched in eicosapentaenoic acid (EPA) significantly increased UCP1 and other thermogenic markers in BAT. It is hypothesized that these effects are mediated through UCP1-dependent regulation. Methods and results Wild-type (WT) and UCP1 knockout (KO) B6 male mice were housed at thermoneutrality and fed a HF diet, without or with eicosapentaenoic acid (EPA)-enriched fish oil. HF-fed KO mice were heavier and had higher BAT lipid content than other groups. Protective effects of EPA in WT, previously observed at 22 degrees C (reduced adiposity, improved glucose tolerance, and increased UCP1), disappeared at thermoneutrality. Mitochondrial proteins, cytochrome c oxidase subunit 1 (COX I), COX I, II, and IV were reduced in the KO mice compared to WT. Unexpectedly, EPA attenuated weight and fat mass gain and improved glucose tolerance in the KO mice. Finally, EPA increased BAT peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC1 alpha) protein and gene expression, and whole-body oxygen consumption in KO mice, consistent with increased mitochondria DNA (mtDNA)/nuclear DNA (nucDNA) ratio. Conclusions EPA rescued the weight gain and glucose intolerance in UCP1 KO mice at thermoneutrality, independent of UCP1; these effects may be mediated in part via increased oxygen consumption and BAT PGC1 alpha. (AU)

Processo FAPESP: 15/19530-5 - Caracterização do envolvimento do sensor de nutrientes mTOR no desenvolvimento de doenças metabólicas crônicas associadas à obesidade
Beneficiário:William Tadeu Lara Festuccia
Linha de fomento: Auxílio à Pesquisa - Temático