Determination of Short Chain Fatty Acids in Mice Feces by Capillary Electrophoresis

Autor(es)

Marques, Leticia A. ^[1] ; Cazarin, Cinthia B. B. ^[2] ; Bicas, Juliano ^[2] ; Marostica Junior, Mario R. ^[2] ; Carrilho, Emanuel ^{[1, 3]} ; Bogusz Junior, Stanislau ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim Sao Carlos IQSC, BR-13566590 Sao Carlos, SP - Brazil

^[2] Univ Estadual Campinas UNICAMP, FEA, CP 6154, BR-13083970 Campinas, SP - Brazil

^[3] Inst Nacl Ciencia & Tecnol Bioanalit INCTBio, CP 6154, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 30, n. 6, p. 1326-1333, JUN 2019.
• Citações Web of Science: 0

Resumo

Short-chain fatty acids (SCFA) are compounds produced during fermentation of gut microbiota. Acetic, propionic and butyric acids are the most important SCFA produced from non-digestible foods. We developed and validated a rapid and low-cost capillary electrophoretic (CE) method for determination of acetic, propionic and butyric acids in mice feces (100 mg of sample). Electrophoretic separation was performed for SCFA determination in feces samples during 10 min. The method showed good linearity for all analytes (determination coefficient, r2 > 0.98); recovery from 74.1 to 109.8%, while the intra- and interday precision essays were suitable (relative standard deviation (RSD) < 10%); and limits of detection and quantification of 0.13-0.43 mM for acetic acid, 0.09-0.29 mM for propionic acid and 0.03-0.09 mM for butyric acid. Real samples of mice dextran sulfate sodium-induced colitis feces treated with jabuticaba (Plinia cauliflora) aqueous extract were performed successfully. (AU)

• Processo FAPESP: 15/50333-1 - Cobra: nova plataforma de descoberta de compostos para identificação rápida e de baixo custo de bioativo com efeitos benéficos originários de plantas brasileiras
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• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica
• Beneficiário: Marco Aurelio Zezzi Arruda
• Modalidade de apoio: Auxílio à Pesquisa - Temático