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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Determination of Short Chain Fatty Acids in Mice Feces by Capillary Electrophoresis

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Author(s):
Marques, Leticia A. [1] ; Cazarin, Cinthia B. B. [2] ; Bicas, Juliano [2] ; Marostica Junior, Mario R. [2] ; Carrilho, Emanuel [1, 3] ; Bogusz Junior, Stanislau [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Inst Quim Sao Carlos IQSC, BR-13566590 Sao Carlos, SP - Brazil
[2] Univ Estadual Campinas UNICAMP, FEA, CP 6154, BR-13083970 Campinas, SP - Brazil
[3] Inst Nacl Ciencia & Tecnol Bioanalit INCTBio, CP 6154, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 30, n. 6, p. 1326-1333, JUN 2019.
Web of Science Citations: 0
Abstract

Short-chain fatty acids (SCFA) are compounds produced during fermentation of gut microbiota. Acetic, propionic and butyric acids are the most important SCFA produced from non-digestible foods. We developed and validated a rapid and low-cost capillary electrophoretic (CE) method for determination of acetic, propionic and butyric acids in mice feces (100 mg of sample). Electrophoretic separation was performed for SCFA determination in feces samples during 10 min. The method showed good linearity for all analytes (determination coefficient, r2 > 0.98); recovery from 74.1 to 109.8%, while the intra- and interday precision essays were suitable (relative standard deviation (RSD) < 10%); and limits of detection and quantification of 0.13-0.43 mM for acetic acid, 0.09-0.29 mM for propionic acid and 0.03-0.09 mM for butyric acid. Real samples of mice dextran sulfate sodium-induced colitis feces treated with jabuticaba (Plinia cauliflora) aqueous extract were performed successfully. (AU)

FAPESP's process: 15/50333-1 - COBRA: a novel compound discovery pipeline for rapid and cost-effective identification of bioactives with health beneficial effects in Brazilian crops
Grantee:Glaucia Maria Pastore
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
Grantee:Marco Aurelio Zezzi Arruda
Support Opportunities: Research Projects - Thematic Grants