The presence of osteocalcin, osteopontin and reactive oxygen species-positive cells in pulp tissue after dental bleaching

Autor(es)

Benetti, F. ^[1] ; Briso, A. L. F. ^[2] ; Carminatti, M. ^[1] ; de Araujo Lopes, J. M. ^[1] ; Barbosa, J. G. ^[1] ; Ervolino, E. ^[3] ; Gomes-Filho, J. E. ^[1] ; Cintra, L. T. A. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ Unesp, Sch Dent, Dept Endodont, Aracatuba, SP - Brazil

^[2] Sao Paulo State Univ Unesp, Sch Dent, Dept Restorat Dent, R Jose Bonifacio 1193, Aracatuba, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Endodontic Journal; v. 52, n. 5, p. 665-675, MAY 2019.
• Citações Web of Science: 1

Resumo

Aim To analyse the influence of H2O2 on pulp repair through osteocalcin and osteopontin immunolabelling and in cellular defence by using the antireactive oxygen species (ROS) antibody. Methodology The maxillary molars of 50 rats were treated with 35% H2O2 (Ble groups) or placebo gel (control groups). At 0 h and 2, 7, 15 and 30 days (n = 10 hemimaxillae), the rats were killed and pulp tissue was evaluated using inflammation and immunolabelling scores (osteocalcin/osteopontin); ROS-positive cells were counted. Paired t-test and Wilcoxon signed-rank test were used (P < 0.05). Results The Ble group had necrosis in the coronal pulp at 0 h and in the occlusal third of the coronal pulp at 2 days; at 7, 15 and 30 days, no inflammation was noted similar to the controls (P > 0.05). Osteocalcin was absent in the Ble at 0 h, moderate at 2 days and increased thereafter, differing from the controls at all two periods (P < 0.05). Osteopontin was higher principally at 7 and 15 days in Ble groups, but differing with control groups from 2 days after bleaching (P < 0.05). The Ble group had more ROS-positive cells in the pulp at 7 and 15 days (P < 0.05). Tertiary dentine was observed at 7 days, increasing thereafter (P < 0.05). Conclusions Post-bleaching pulp repair was associated with increased osteocalcin over time. Osteopontin also participated in this process, and anti-ROS was involved in cellular defence against H2O2. (AU)

• Processo FAPESP: 15/10825-2 - Influência do Peróxido de Hidrogênio sobre a diferenciação celular e a mineralização do tecido pulpar após procedimento clareador. Estudo histológico e imunoistoquímico.
• Beneficiário: Francine Benetti
• Modalidade de apoio: Bolsas no Brasil - Doutorado