Effect of intermittent teriparatide (PTH 1-34) on the alveolar healing process in orchiectomized rats

Autor(es)

de Oliveira, Danila ^[1] ; Puttini, Igor de Oliveira ^{[2, 3]} ; Silva Gomes-Ferreira, Pedro Henrique ^{[2, 3]} ; Palin, Leticia Pitol ^[1] ; Matsumoto, Mariza Akemi ^[1] ; Okamoto, Roberta ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Basic Sci, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Surg, Aracatuba, SP - Brazil

^[3] Univ Estadual Paulista UNESP, Aracatuba Dent Sch, Integrated Clin, Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; v. 23, n. 5, p. 2313-2322, MAY 2019.
• Citações Web of Science: 1

Resumo

ObjectiveTo evaluate intermittent teriparatide therapy's influence on the alveolar healing process in rats with induced osteoporosis.Materials and methodsFifty-four male rats were divided into three groups: fictitious surgery (SHAM), bilateral orchiectomy (ORQ NT), and bilateral orchiectomy treated with teriparatide (ORQ TERI). Right upper incisor extraction was performed. After 14 and 42days, the rats were euthanized. Immunolabeling analysis was performed in order to characterize bone turnover through Wnt, alcaline phosphatase, osteocalcin, and TRAP presence in tissue; micro-CT analysis was performed in order to determine the bone volume fraction (BV/TV), trabecular thickness, separation, and number (Tb.Th, Tb.Sp, Tb.N). For the micro-CT data, statistical analysis was performed through one-way ANOVA and Tukey post-test, for parametrical data, with significance level adopted in p<0.05. Days, Wnt, alkaline phosphatase, and osteocalcin revealed more labeling for ORQ TERI and less for TRAP. For the Tb.N in the ORQ NT group was 0.496mm, a significant statistical difference was observed between the groups of ORQ NT and ORQ TERI (p<0.05). For the BV/TV, Tb.Sp, and Tb.Th parameters, no significant statistical difference was observed among the three experimental groups (p>0.05).ConclusionsTreatment with intermittent teriparatide reverted impairment in the metabolism of repairing bone tissue in orchiectomized animals.Clinical relevanceCases of decreased bone density such as osteoporosis can lead to delayed alveolar repair process. PTH 1-34 has been shown to be a medication that improves this repair, making bone of low quality into one with normal features. (AU)

• Processo FAPESP: 15/14688-0 - Caracterização do tecido ósseo formado durante o processo de reparo alveolar e a osseointegração em ratos submetidos a terapia anti-osteoporose. Aspectos histológicos, histométricos, imunoistoquimicos, moleculares e tridimensionais.
• Beneficiário: Roberta Okamoto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/20203-9 - Efeito da Teriparatida sobre o processo de reparo alveolar em ratos orquiectomizados: análise imunoistoquímica, histométrica e microtomográfica
• Beneficiário: Danila de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica