In Vitro Cellular Division of Trypanosoma abeli Reveals Two Pathways for Organelle Replication

Autor(es)

Borges, Alyssa R. ^[1] ; Toledo, Daniel A. ^[2] ; Fermino, Bruno R. ^[3] ; de Oliveira, Jose Carlos ^[4] ; Silber, Ariel Mariano ^[5] ; Elias, Maria Carolina ^[6] ; D'Avila, Heloisa ^[2] ; Scopel, Kezia K. G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Juiz de Fora, Inst Biol Sci, Dept Parasitol Microbiol & Immunol, Parasitol Lab, Rua Jose Lourenco Kelmer S-N, Campus Univ, BR-36036900 Juiz De Fora, MG - Brazil

^[2] Univ Fed Juiz de Fora, Inst Biol Sci, Dept Biol, Lab Cellular Biol, Rua Jose Lourenco Kelmer S-N, Campus Univ, BR-36036900 Juiz De Fora, MG - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Ave Prof Lineu Prestes 1374 Cidade Univ, BR-05508900 Sao Paulo, SP - Brazil

^[4] Univ Fed Juiz de Fora, Inst Biol Sci, Dept Zool, Rua Jose Lourenco Kelmer S-N, Campus Univ, BR-36036900 Juiz De Fora, MG - Brazil

^[5] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, LaBTryps, Ave Prof Lineu Prestes 1374 Cidade Univ, BR-05508000 Sao Paulo, SP - Brazil

^[6] Inst Butantan, Ctr Toxins Immune Response & Cell Signaling CeTIC, Lab Especial Ciclo Celular, Ave Vital Brasil 1500 Butanta, BR-05503900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Eukaryotic Microbiology; v. 66, n. 3, p. 385-392, MAY-JUN 2019.
• Citações Web of Science: 1

Resumo

Since the observation of the great pleomorphism of fish trypanosomes, in vitro culture has become an important tool to support taxonomic studies investigating the biology of cultured parasites, such as their structure, growth dynamics, and cellular cycle. Relative to their biology, ex vivo and in vitro studies have shown that these parasites, during the multiplication process, duplicate and segregate the kinetoplast before nucleus replication and division. However, the inverse sequence (the nucleus divides before the kinetoplast) has only been documented for a species of marine fish trypanosomes on a single occasion. Now, this previously rare event was observed in Trypanosoma abeli, a freshwater fish trypanosome. Specifically, from 376 cultured parasites in the multiplication process, we determined the sequence of organelle division for 111 forms; 39% exhibited nucleus duplication prior to kinetoplast replication. Thus, our results suggest that nucleus division before the kinetoplast may not represent an accidental or erroneous event occurring in the main pathway of parasite reproduction, but instead could be a species-specific process of cell biology in trypanosomes, such as previously noticed for Leishmania. This ``alternative{''} pathway for organelle replication is a new field to be explored concerning the biology of marine and freshwater fish trypanosomes. (AU)

• Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
• Beneficiário: Hugo Aguirre Armelin
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs