Enriched microbial consortia for dark fermentation of sugarcane vinasse towards value-added short-chain organic acids and alcohol production

Autor(es)

Moraes, Bruna de Souza ^[1] ; dos Santos, Graciete Mary ^[2] ; Delforno, Tiago Palladino ^[3] ; Fuess, Lucas Tadeu ^[4] ; da Silva, Ariovaldo Jose ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Interdisciplinary Ctr Energy Planning, NIPE, Rua Cora Coralina 330, BR-13083896 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Sch Agr Engn, FEAGRI, Av Candido Rondon 501, BR-13083875 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Res Ctr Chem Biol & Agr, Microbial Resources Div, CPQBA, Av Alexandre Cazelatto 999, BR-13148218 Paulinia, SP - Brazil

^[4] Univ Sao Paulo, Polytech Sch, Dept Chem Engn, DEQ, EPUSP, Av Prof Lineu Prestes 580, BR-05508000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Bioscience and Bioengineering; v. 127, n. 5, p. 594-601, MAY 2019.
• Citações Web of Science: 2

Resumo

The role of sugarcane vinasse as a nutrient source and the impacts of different inoculum pretreatment methods (acid thermal and thermal treatment) were assessed in acidogenic systems aiming to produce value-added short-chain organic acids (SCOA) and alcohols. In-depth microbiome characterization was also conducted by high-throughput sequencing of the 16S rRNA gene using the Miseq Illumina platform. SCOA production was 473 % higher in vinassefed reactors, with isobutyric (up to 10,3 g L-1) and butyric (up to 10.6 g L-1) acids as the primary metabolites most likely resulting from lactate conversion. Ethanol comprised the main product from solventogenic pathways in all conditions, with values ranging between 2.7 and 5.2 g L-1,whereas no butanol was detected. Microbial analyses revealed high relative abundance values for the Clostridium, Lactobacillus, Bacillus and Ruminococcus genera, with the predominance of the Clostridium genus (17%) in acid-thermal treatment reactors and the Lactobacillus genus (37%) in thermal treatment reactors. Overall, vinasse proved to be a suitable substrate for value-added SCOA production, which characterizes a potential management approach to this wastewater stream. In this sense, the biochemical production of butyrate from vinasse could diversify the product portfolio of sugarcane biorefineries, also minimizing bioenergy losses by converting residual carbon fractions. (C) 2018, The Society for Biotechnology, Japan. All rights reserved. (AU)

• Processo FAPESP: 12/09785-8 - Digestão anaeróbia de águas residuárias direcionada para a produção de butanol e etanol
• Beneficiário: Ariovaldo José da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 16/16438-3 - Produção de biogás em biorrefinarias de cana-de-açúcar integradas de 1ª e 2ª geração: aspectos fundamentais e operacionais do processo
• Beneficiário: Bruna de Souza Moraes
• Modalidade de apoio: Auxílio à Pesquisa - Regular