A Recombinant Enolase-Montanide PetGel A Vaccine Promotes a Protective Th1 Immune Response against a Highly Virulent Sporothrix schenckii by Toluene Exposure

Autor(es)

Tellez-Martinez, Damiana ^[1] ; Portuondo, Deivys Leandro ^[1] ; Loesch, Maria Luiza ^[1] ; Batista-Duharte, Alexander ^[1] ; Carlos, Iracilda Zeppone ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Sch Pharmaceut Sci, Dept Clin Anal, UNESP, BR-14800903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PHARMACEUTICS; v. 11, n. 3 MAR 2019.
• Citações Web of Science: 2

Resumo

The effect of vaccination in fungal strains that suffered changes in their virulence by exposure to environmental contaminants is largely known. Growing reports of resistance to antifungal drugs and the emergence of new highly virulent strains, possibly acquired in the environment, prompt the design of new vaccines able to prevent and combat emerging mycotic diseases. In this study, we evaluated the protective capacity of an enolase-based vaccine and Montanide PetGel A (PGA) as an adjuvant against S. schenckii with increased virulence by exposure to toluene. The adjuvanted vaccine induced a strong specific Th1 response and protective immunity against a challenge with either wildtype or toluene-adapted S. schenckii in Balb/c mice. This study highlights the role of the adjuvant PGA driving the quality of the anti-sporothrix immunity and the key component in the vaccine efficacy. (AU)

• Processo FAPESP: 17/26774-3 - Imunogenicidade e eficácia pré-clinica de um candidato vacinal de enolase recombinante contra Sporothrix schenckii e Sporothrix brasiliensis
• Beneficiário: Iracilda Zeppone Carlos
• Modalidade de apoio: Auxílio à Pesquisa - Regular