A Recombinant Enolase-Montanide PetGel A Vaccine Promotes a Protective Th1 Immune Response against a Highly Virulent Sporothrix schenckii by Toluene Exposure

Author(s)

Tellez-Martinez, Damiana ^[1] ; Portuondo, Deivys Leandro ^[1] ; Loesch, Maria Luiza ^[1] ; Batista-Duharte, Alexander ^[1] ; Carlos, Iracilda Zeppone ^[1]

Affiliation

^[1] Sao Paulo State Univ, Sch Pharmaceut Sci, Dept Clin Anal, UNESP, BR-14800903 Araraquara, SP - Brazil

• Document type: Journal article
• Source: PHARMACEUTICS; v. 11, n. 3 MAR 2019.
• Web of Science Citations: 2

Abstract

The effect of vaccination in fungal strains that suffered changes in their virulence by exposure to environmental contaminants is largely known. Growing reports of resistance to antifungal drugs and the emergence of new highly virulent strains, possibly acquired in the environment, prompt the design of new vaccines able to prevent and combat emerging mycotic diseases. In this study, we evaluated the protective capacity of an enolase-based vaccine and Montanide PetGel A (PGA) as an adjuvant against S. schenckii with increased virulence by exposure to toluene. The adjuvanted vaccine induced a strong specific Th1 response and protective immunity against a challenge with either wildtype or toluene-adapted S. schenckii in Balb/c mice. This study highlights the role of the adjuvant PGA driving the quality of the anti-sporothrix immunity and the key component in the vaccine efficacy. (AU)

• FAPESP's process: 17/26774-3 - Immunogenicity and preclinical efficacy of a recombinant enolase vaccine candidate against Sporothrix schenckii and Sporothrix brasiliensis
• Grantee: Iracilda Zeppone Carlos
• Support Opportunities: Regular Research Grants