Influence of Sugarcane Genotype and Soil Moisture Level on the Arbuscular Mycorrhizal Fungi Community

Autor(es)

da Silva Barros, Timoteo Herculino ^[1] ; de Araujo Pereira, Arthur Prudencio ^[2] ; de Souza, Adijailton Jose ^[2] ; Ribeiro, Nathalia Lopes ^[1] ; Bran Nogueira Cardoso, Elke Jurandy ^[2] ; Coelho, Rubens Duarte ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Agr Syst Engn Dept, Piracicaba - Brazil

^[2] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Soil Sci Dept, Piracicaba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SUGAR TECH; v. 21, n. 3, p. 505-513, JUN 2019.
• Citações Web of Science: 1

Resumo

The relationship between sugarcane genotype and symbiosis with arbuscular mycorrhizal fungi (AMF) remains poorly understood, especially regarding different soil moisture levels. Our objective was to evaluate the effect of soil moisture on the AMF community structure, spore abundance and colonization ratio in a plantation with eight sugarcane genotypes (CTC15, CTC17, RB867515, RB92579, RB931011, RB966928, IAC5000 and NCo376). The study was carried out in Piracicaba, SAo Paulo and Brazil in an experimental plot setup in a randomized block design, with three replicates (blocks). We collected soil and root samples in a greenhouse experiment under two water replenishment levels: 100 and 50% of soil moisture at field capacity ((FC)). We extracted spores and assessed the AMF root colonization ratio by using specific dyes and determining the percentage of root length colonized in the different sugarcane genotypes. In addition, we evaluated the AMF community structure by PCR and denaturing gradient gel electrophoresis. In general, the spore abundance and root colonization ratio were higher in all varieties at 100% (FC). However, the IAC5000 and RB966928 genotypes showed higher colonization levels even at 50% (FC). The AMF community structure was also influenced by soil water levels with group separations across 100 and 50% (FC). Sugarcane productivity as measured by stalk plus root dry mass was positively correlated with AMF colonization rates in 100% (FC). Thus, the water replenishment levels used in sugarcane cultivation can influence spore abundance, colonization ratios and AMF community structure in the soil. The selection of a sugarcane genotype with greater AMF association under low water replenishment levels may be a primary factor in growing sugarcane in areas with low water availability. (AU)

• Processo FAPESP: 12/50083-7 - Produtividade da água em biomassa e energia para variedades de cana-de-açúcar em função da disponibilidade hídrica no solo: experimentação e simulação
• Beneficiário: Rubens Duarte Coelho
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - PITE