Effect of metronidazole ophthalmic solution on corneal neovascularization in a rat model

Autor(es)

Yokoay Claros-Chacaltana, Flor Diana ^[1] ; Aldrovani, Marcela ^[1] ; Kobashigawa, Karina Kamachi ^[1] ; Martinez Padua, Ivan Ricardo ^[1] ; Valdetaro, Gisele Pereira ^[1] ; Franchi de Barros Sobrinho, Alexandre Augusto ^[1] ; Morato Abreu, Thais Guimaraes ^[1] ; Laus, Jose Luiz ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Agr & Veterinarian Sci FCAV, Dept Vet Clin & Surg, Ophthalmol Unit, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL OPHTHALMOLOGY; v. 39, n. 5, p. 1123-1135, MAY 2019.
• Citações Web of Science: 0

Resumo

PurposeTo evaluate the effect of metronidazole ophthalmic solutions on corneal neovascularization (CNV) in a rat model.MethodsA chemical burn was created in the right central cornea of 40 rats. Animals were randomized and distributed into four study groups (n=10 rats per group) designated Met\_0.1%, Met\_0.5%, sham, and untreated groups. Chemical-burned corneas in the Met\_0.1% and Met\_0.5% groups received ophthalmic solutions of 0.1 and 0.5% metronidazole, respectively. Corneas in the sham group received phosphate-buffered saline (metronidazole diluent). All treated eyes received ophthalmic solution at intervals of 6h, for up to 30days. Untreated corneas received no treatment. CNV was evaluated postinjury using corneal photographs at different evaluation time points. The main CNV outcome measures were: burn intensity, index of CNV, and percentage of vascularized corneal area. Five rats from each group were euthanized, on days 15 and 30; the samples were collected for histological analyses. Differences with P<0.05 were considered significant.ResultsCNV was observed in the eyes from day 7 postinjury. However, the indices of CNV for the Met\_0.1% and Met\_0.5% groups were smaller than those for the sham and untreated groups (P<0.05). Furthermore, corneas treated with 0.1 or 0.5% metronidazole had smaller vascularized areas compared to control corneas. On histological study, the presence of blood vessels confirmed clinical outcomes.ConclusionsRegular instillation of 0.1 or 0.5% metronidazole had a significant inhibitory effect for CNV on chemical burns induced in a rat model. (AU)

• Processo FAPESP: 12/17308-5 - Supraorganização cromatínica, conteúdo de DNA, concentração de glicoproteínas nucleares e avaliação imunofenotípica de células epiteliais límbicas expandidas sobre membrana amniótica intacta ou desepitelizada versus membrana de fucoidam-alginato-quitosana
• Beneficiário: Marcela Aldrovani Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/51773-4 - Dois projetos de pesquisa: 1) Lente intraocular dobravel na profilaxia e Nd:YAG laser no manejo da opacidade de cápsula posterior pós-facoemulsificação em cães, e 2) Avaliação da técnica V-Prechop de nucleodissecção, na facoemulsificação em cães
• Beneficiário: José Luiz Laus
• Modalidade de apoio: Auxílio à Pesquisa - Regular