Novel insights on the versatility of biohydrogen production from sugarcane vinasse via thermophilic dark fermentation: Impacts of pH-driven operating strategies on acidogenesis metabolite profiles

Autor(es)

Fuess, Lucas Tadeu ^{[1, 2]} ; Zaiat, Marcelo ^[1] ; Oller do Nascimento, Claudio Augusto ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo LPB EESC USP, Sao Carlos Sch Engn, Biol Proc Lab, Ave Joao Dagnone 1100, BR-13563120 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo DEQ EP USP, Polytech Sch, Dept Chem Engn, Ave Prof Lineu Prestes 580, Bloco 18 Conjunto Quim, BR-05508000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Bioresource Technology; v. 286, AUG 2019.
• Citações Web of Science: 3

Resumo

An innovative application of the anaerobic structured-bed reactor (AnSTBR) in thermophilic dark fermentation of sugarcane vinasse targeting biohydrogen (bioH(2)) production was assessed. A detailed metabolite monitoring program identified the major substrates and primary metabolic pathways within the system. Increasing the applied organic loading rate positively affected bioH(2) production, reaching 2074 N mL-H-2 L-1 d(-1) and indicating an optimal load of approximately 70 kg-COD m(-3) d(-1). Controlling the fermentation pH (5.0-5.5) was the primary strategy to maintain bioH(2)-producing conditions, offsetting negative impacts associated with the compositional variability of vinasse. Metabolic correlations pointed out lactate as the primary substrate for bioH(2) production, indicating its accumulation as evidence of impaired reactors. The versatility of the acidogenic system was confirmed by identifying three major metabolic pathways according to the pH, i.e., lactate-producing (pH < 5.0), bioH(2)-/butyrate-producing (pH = 5.0-5.5) and bioH(2)-producing/sulfate-reducing (pH > 6.0) systems, which enables managing the operation of the reactors for diversified purposes in practical aspects. (AU)

• Processo FAPESP: 15/50684-9 - Sustainable gas pathways for Brazil: from microcosm to macrocosm
• Beneficiário: Reinaldo Giudici
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/00080-5 - Potencialização da produção de energia a partir da cana-de-açúcar em biorrefinarias de primeira geração: biodigestão como etapa central de conversão
• Beneficiário: Lucas Tadeu Fuess
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/06246-7 - Aplicação do conceito de biorrefinaria a estações de tratamento biológico de águas residuárias: o controle da poluição ambiental aliado à recuperação de matéria e energia
• Beneficiário: Marcelo Zaiat
• Modalidade de apoio: Auxílio à Pesquisa - Temático