| Texto completo | |
| Autor(es): |
Número total de Autores: 2
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| Afiliação do(s) autor(es): | [1] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, BR-13083862 Campinas, SP - Brazil
Número total de Afiliações: 1
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| Tipo de documento: | Artigo Científico |
| Fonte: | International Journal of Biological Macromolecules; v. 131, p. 510-519, JUN 15 2019. |
| Citações Web of Science: | 1 |
| Resumo | |
Optical anisotropy properties and infrared spectra of collagen and DNA as individual entities have been widely described. However, they have not been studied in collagen-DNA complex biofilms. We investigated whether collagen type I and DNA birefringence evaluated using polarization microscopy, and the vibrational patterns of their chemical groups identified in Fourier transform-infrared (FT-IR) spectra are retained in collagen-DNA complexes. Collagen gel rods extruded into a DNA saline solution and films dried from drops using mixtures of a 10-mu L (14 mu g) collagen solution and a 50-mu L (305 mu g) DNA solution and of a 50-mu L (70 mu g) collagen solution and a 10-mu L (61 mu g) DNA solution were used. Changes in the collagen fibers' stereoarrangement occurred in the gel rods interacting with the DNA. Even when the DNA concentration (305 mu g) was significantly higher than the collagen concentration (14 mu g) and the DNA played the main role in establishing the overall oriented geometry in the collagen-DNA films (intertwisted fibrillary structure and negative birefringence), some collagen markers were present in the FT-IR spectra of the complex. When collagen and DNA concentrations were similar, DNA denaturation may occur. Present observations suggest formation of collagen-DNA complexes even if highly organized collagen macromolecules are offered to DNA. (C) 2019 Elsevier B.V. All rights reserved. (AU) | |
| Processo FAPESP: | 07/58251-8 - Estrutura 3-D helicoidal, propriedades anisotrópicas ópticas e transformada de Fourier-infravermelho de fibras de colágeno em Chordae tendinae |
| Beneficiário: | Benedicto de Campos Vidal |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |