Cyanobacterial biodiversity of semiarid public drinking water supply reservoirs assessed via next-generation DNA sequencing technology

Autor(es)

Lorenzi, Adriana Sturion ^{[1, 2]} ; Chia, Mathias Ahii ^{[2, 3]} ; Cardoso Lopes, Fabyano Alvares ^{[1, 4]} ; Silva, Genivaldo Gueiros Z. ^[1] ; Edwards, Robert A. ^{[5, 1]} ; Bittencourt-Oliveira, Maria do Carmo ^[2]

Afiliação do(s) autor(es)

^[1] San Diego State Univ, Computat Sci Res Ctr, San Diego, CA 92182 - USA

^[2] Univ Sao Paulo, Luiz Queiroz Coll Agr, Dept Biol Sci, Lab Cyanobacteria, Piracicaba, SP - Brazil

^[3] SUNY Stony Brook, Sch Marine & Atmospher Sci, Southampton Campus, Stony Brook, NY 11794 - USA

^[4] Univ Brasilia UNB, Dept Cell Biol, Lab Enzymol, Brasilia, DF - Brazil

^[5] San Diego State Univ, Dept Comp Sci, San Diego, CA 92182 - USA

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MICROBIOLOGY; v. 57, n. 6, p. 450-460, JUN 2019.
• Citações Web of Science: 0

Resumo

Next-generation DNA sequencing technology was applied to generate molecular data from semiarid reservoirs during well-defined seasons. Target sequences of 16S-23S rRNA ITS and cpcBA-IGS were used to reveal the taxonomic groups of cyanobacteria present in the samples, and genes coding for cyanotoxins such as microcystins (mcyE), saxitoxins (sxtA), and cylindrospermopsins (cyrJ) were investigated. The presence of saxitoxins in the environmental samples was evaluated using ELISA kit. Taxonomic analyses of high-throughput DNA sequencing data showed the dominance of the genus Microcystis in Mundau reservoir. Furthermore, it was the most abundant genus in the dry season in Ingazeira reservoir. In the rainy season, 16S-23S rRNA ITS analysis revealed that Cylindrospermopsis raciborskii comprised 46.8% of the cyanobacterial community in Ingazeira reservoir, while the cpcBAIGS region revealed that C. raciborskii (31.8%) was the most abundant taxon followed by Sphaerospermopsis aphanizomenoides (17.3%) and Planktothrix zahidii (16.6%). Despite the presence of other potential toxin-producing genera, the detected sxtA gene belonged to C. raciborskii, while the mcyE gene belonged to Microcystis in both reservoirs. The detected mcyE gene had good correlation with MC content, while the amplification of the sxtA gene was related to the presence of STX. The cyrJ gene was not detected in these samples. Using DNA analyses, our results showed that the cyanobacterial composition of Mundau reservoir was similar in successive dry seasons, and it varied between seasons in Ingazeira reservoir. In addition, our data suggest that some biases of analysis influenced the cyanobacterial communities seen in the NGS output of Ingazeira reservoir. (AU)

• Processo FAPESP: 13/11306-3 - Efeitos de anatoxina-a e cilindrospermopsina em diferentes concentrações de nitrogênio e irradiância na fisiologia de Microcystis aeruginosa (Cyanobacteria)e Scenedesmus acuminatus (Chlorophyta)
• Beneficiário: Mathias Ahii Chia
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/01913-2 - Metagenômica comparativa de florações de cianobactérias em reservatórios de água do Estado de Pernambuco.
• Beneficiário: Adriana Sturion Lorenzi
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores
• Processo FAPESP: 13/15296-2 - Metagenômica comparativa de florações de cianobactérias em reservatórios de água do estado de Pernambuco
• Beneficiário: Adriana Sturion Lorenzi
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores